Vanhamme L, Rolin S, Szpirer C
Département de Biologie Moléculaire, Université Libre de Bruxelles, Rhode-St-Genèse, Belgium.
Exp Cell Res. 1989 Jan;180(1):297-301. doi: 10.1016/0014-4827(89)90234-6.
In order to study the effects of an activated H-ras-1 oncogene on gap-junctional intercellular communication, we introduced the EJ/T24 H-ras-1 oncogene into cells of the epithelial Clone 9-3 cell line. Gap-junctional intercellular communication was significantly reduced in H-ras-1-transformed Clone 9-3 derivatives; this result shows that transformation by the activated H-ras-1 oncogene can inhibit gap-junctional intercellular communication. We postulate that the activated H-ras-1 oncogene product could mediate this effect through a change in the phosphorylation of the major gap-junction protein.
为了研究激活的H-ras-1癌基因对间隙连接细胞间通讯的影响，我们将EJ/T24 H-ras-1癌基因导入上皮克隆9-3细胞系的细胞中。在H-ras-1转化的克隆9-3衍生物中，间隙连接细胞间通讯显著减少；这一结果表明，激活的H-ras-1癌基因转化可抑制间隙连接细胞间通讯。我们推测，激活的H-ras-1癌基因产物可能通过改变主要间隙连接蛋白的磷酸化来介导这种效应。
