Ahmed Furqan, Roy Choudhury Namita, Dutta Naba K, Zou Linda, Zannettino Andrew
a Ian Wark Research Institute , University of South Australia, Mawson Lakes Campus , Mawson lakes , South Australia, Australia.
J Biomater Sci Polym Ed. 2014;25(18):2023-41. doi: 10.1080/09205063.2014.968018. Epub 2014 Oct 31.
In this research, nanofibrous 3D tubular (~4-mm-diameter tube) scaffolds of poly (vinylidene fluoride-co-hexafluoropropylene) were fabricated by electrospinning. The role of surface charge in the success of these scaffolds for potential small-diameter artificial vascular grafts has been investigated using streaming potential study. Prior to endothelial cell culture, surface properties such as wettability and the surface charge of these tubular scaffolds were evaluated using unmodified and fibrinogen-adsorbed surfaces to understand their interaction with surrounding environment. The tubular scaffolds constructed using electrospinning show similar mechanical properties such as tensile strength and elastic modulus as those of native vessels. Whilst endothelial cell proliferation on unmodified tubes, as analysed by scanning electron microscopy, was found to be moderate, a simple process of dynamic fibrinogen adsorption was seen to enhance the endothelialisation of these tubular grafts. The high negative zeta potential values, high strength, robustness and structural reliability of the scaffolds represent them to be promising biomaterials for vascular graft applications.
在本研究中，通过静电纺丝制备了聚（偏二氟乙烯 - 共 - 六氟丙烯）的纳米纤维三维管状（直径约4毫米的管子）支架。利用流动电势研究，探讨了表面电荷在这些支架用于潜在小直径人工血管移植物成功中的作用。在内皮细胞培养之前，使用未修饰和纤维蛋白原吸附的表面评估这些管状支架的表面性质，如润湿性和表面电荷，以了解它们与周围环境的相互作用。通过静电纺丝构建的管状支架显示出与天然血管相似的机械性能，如拉伸强度和弹性模量。通过扫描电子显微镜分析发现，未修饰管上的内皮细胞增殖适中，而简单的动态纤维蛋白原吸附过程被认为可增强这些管状移植物的内皮化。支架的高负zeta电位值、高强度、坚固性和结构可靠性表明它们是用于血管移植物应用的有前景的生物材料。
