Hakem R, Le Bouteiller P, Barad M, Trujillo M, Mercier P, Wietzerbin J, Lemonnier F A
Centre d'Immunologie, INSERM-CNRS, Marseille-Luminy, France.
J Immunol. 1989 Jan 1;142(1):297-305.
The regulation by IFN of the expression of HLA-B7 and HLA-A3 class I molecules was studied in Jurkat human T lymphoma cells, HHK EBV-transformed human B lymphocytes, and murine HLA-B7 HLA-A3 co-transfected L fibroblasts. Jurkat cells express constitutively low level of HLA class I molecules and treatment with human IFN resulted in preferential increase of the expression of HLA-B7 molecules, the expression of the HLA-A3 molecules being relatively unchanged. Similar treatment of HHK cells, which express constitutively large amount of HLA class I molecules, resulted in a marginal increase of the expression of both HLA-B7 and HLA-A3 molecules. HLA-B7 HLA-A3 co-transfected L cells express relatively low level of HLA class I molecules, expression of both however was significantly increased after treatment with murine INF-alpha, the augmentation being more accentuated for HLA-B7 molecules. In all cases, variations of cell surface expression were related to parallel modifications of the level of HLA-B7 and HLA-A3 RNA transcripts. Important nucleotide differences exist between the IFN consensus sequences associated with the HLA-B7 and HLA-A3 class I genes. Using oligonucleotides corresponding to these sequences two patterns of retarded bands were observed by the gel mobility shift assay, suggesting that the IFN-mediated differential regulation of the expression of the HLA-B7 and HLA-A3 genes could be due to different nuclear regulatory factors.
在人Jurkat T淋巴瘤细胞、HHK EBV转化的人B淋巴细胞以及鼠源HLA - B7 HLA - A3共转染的L成纤维细胞中研究了干扰素（IFN）对HLA - B7和HLA - A3 I类分子表达的调节作用。Jurkat细胞组成性表达低水平的HLA I类分子，用人干扰素处理后导致HLA - B7分子的表达优先增加，而HLA - A3分子的表达相对不变。对组成性表达大量HLA I类分子的HHK细胞进行类似处理，导致HLA - B7和HLA - A3分子的表达均略有增加。HLA - B7 HLA - A3共转染的L细胞表达相对低水平的HLA I类分子，然而在用鼠源INF - α处理后，两者的表达均显著增加，HLA - B7分子的增加更为明显。在所有情况下，细胞表面表达的变化与HLA - B7和HLA - A3 RNA转录本水平的平行改变相关。与HLA - B7和HLA - A3 I类基因相关的干扰素共有序列之间存在重要的核苷酸差异。使用与这些序列对应的寡核苷酸，通过凝胶迁移率变动分析观察到两种滞后带模式，表明IFN介导的HLA - B7和HLA - A3基因表达的差异调节可能是由于不同的核调节因子所致。
