Chen Xian, He Li-qun
Department of Nephrology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200021,China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2014 Oct;36(5):461-5. doi: 10.3881/j.issn.1000-503X.2014.05.001.
To investigate the mechanism of "Jianpi Qinghua Decoction" (JQD) on renal fibrosis by observing the impact of JQD on serum total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), very-low-density lipoprotein (VLDL), and kidney tumor necrosis factor-Α (TNF-Α) expressions in focal segmental glomerulosclerosis rats induced by nephrectomy combined with adriamycin.
Totally 56 male SD rats were divided into normal group, sham operation group, model group, JQD group, Yiqi Jianpi group, Qingre Huashi group, and Niaoduqing group (all n=8). The model of focal segmental glomerulosclerosis was established by the unilateral nephrectomy and the injection of adriamycin in caudal vein of rat at a dose of 3 mg/kg in the latter 5 groups. JQD, the disassembled prescription of Jianpi Qinghua Decoction (Yiqi Jianpi Decoction and Qingre Huashi Decoction), and Niaoduqing Capsule were administered separately for 8 weeks. The serum TC, TG, LDL, and VLDL levels and the expression of Kidney TNF-Α were determined.
Compared with normal group and sham operation group, the serum TC, TG, LDL, and VLDL levels and the kidney TNF-Α expression in the model group were significantly higher (all P<0.01). Compared with the model group, the JQD group, Qingre Huashi group, and Niaoduqing group had significantly lower serum TC, TG, LDL, and VLDL levels and kidney TNF-Α expression (all P<0.01). Compared with the model group, the Yiqi Jianpi group had significantly lower serum TC ,TG, and VLDL levels (all P<0.01), while the serum LDL level and kidney TNF-Α expression remained unchanged (all P>0.05).
JQD can regulate serum lipids and lower the TNF-Α expression in kidney tissue and thus improve the renal inflammation and relieve renal fibrosis. The heat-clearing and dampness-removing herbs in the prescription play a central role in fighting against renal fibrosis.
通过观察健脾清化方（JQD）对肾切除联合阿霉素诱导的局灶节段性肾小球硬化大鼠血清总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白（LDL）、极低密度脂蛋白（VLDL）及肾组织肿瘤坏死因子-α（TNF-α）表达的影响，探讨健脾清化方抗肾纤维化的作用机制。
将56只雄性SD大鼠分为正常组、假手术组、模型组、健脾清化方组、益气健脾组、清热化湿组和尿毒清组（每组n = 8）。后5组大鼠采用单侧肾切除并尾静脉注射3 mg/kg阿霉素的方法建立局灶节段性肾小球硬化模型。分别给予健脾清化方、其拆方（益气健脾方和清热化湿方）及尿毒清胶囊灌胃8周。检测血清TC、TG、LDL、VLDL水平及肾组织TNF-α表达。
与正常组和假手术组比较，模型组血清TC、TG、LDL、VLDL水平及肾组织TNF-α表达显著升高（均P < 0.01）。与模型组比较，健脾清化方组、清热化湿组和尿毒清组血清TC、TG、LDL、VLDL水平及肾组织TNF-α表达显著降低（均P < 0.01）。与模型组比较，益气健脾组血清TC、TG、VLDL水平显著降低（均P < 0.01），血清LDL水平及肾组织TNF-α表达无明显变化（均P > 0.05）。
健脾清化方可调节血脂，降低肾组织TNF-α表达，从而减轻肾脏炎症，缓解肾纤维化。方中清热利湿药在抗肾纤维化中起核心作用。
