Gidwani Bina, Vyas Amber
a University Institute of Pharmacy, Pt. Ravi Shankar Shukla University , Raipur , Chhattisgarh , India.
Artif Cells Nanomed Biotechnol. 2016;44(2):571-80. doi: 10.3109/21691401.2014.971462. Epub 2014 Nov 3.
The objective of the present study was to prepare solid lipid nanoparticles (SLNs) of altretamine (ALT) by the hot homogenization and ultrasonication method. The study was conducted using the Box-Behnken design (BBD), with a 3(3) design and a total of 17 experimental runs, performed in combination with response surface methodology (RSM). The SLNs were evaluated for mean particle size, entrapment efficiency, and drug-loading. The optimized formulation, with a desirability factor of 0.92, was selected and characterized. In vitro release studies showed a biphasic release pattern from the SLNs for up to 24 h. The results of % EE (93.21 ± 1.5), %DL (1.15 ± 0.6), and mean diameter of (100.6 ± 2.1) nm, were very close to the predicted values.
本研究的目的是通过热均质化和超声处理方法制备六甲蜜胺（ALT）固体脂质纳米粒（SLNs）。该研究采用Box-Behnken设计（BBD），3（3）设计，共进行17次实验运行，并结合响应面法（RSM）。对SLNs的平均粒径、包封率和载药量进行了评估。选择了可取性因子为0.92的优化配方并进行了表征。体外释放研究表明，SLNs在长达24小时内呈现双相释放模式。%EE（93.21±1.5）、%DL（1.15±0.6）和平均直径（100.6±2.1）nm的结果与预测值非常接近。
