Nestel U, Wacker T, Woitzik D, Weckesser J, Kreutz W, Welte W
Institut für Biophysik und Strahlenbiologie der Albert-Ludwigs-Universität, Freiburg i.Br., FRG.
FEBS Lett. 1989 Jan 2;242(2):405-8. doi: 10.1016/0014-5793(89)80511-3.
Porin monomers of the phototrophic bacterium Rhodobacter capsulatus were purified. Crystals were obtained from a solution of porin solubilized with the detergent octyltetraoxyethylene within 5 days using the vapor phase equilibration technique. The crystals were rhombohedral with an edge length of 0.4 mm. The space group was trigonal R3 with unit cell constants of a = b = 95 A, c = 147 A. Reflexions were observed to 3.2 A.
对光合细菌荚膜红假单胞菌的孔蛋白单体进行了纯化。使用气相平衡技术，在5天内从用去污剂辛基四氧乙烯溶解的孔蛋白溶液中获得了晶体。晶体为菱面体，边长0.4毫米。空间群为三方晶系R3，晶胞常数a = b = 95 Å，c = 147 Å。观察到的衍射分辨率为3.2 Å。
