在限定培养基中对人类多能干细胞进行冷冻保存。
Cryopreservation of human pluripotent stem cells in defined medium.
作者信息
Liu Weiwei, Chen Guokai
机构信息
Faculty of Health Sciences, University of Macau, Taipa, Macau, China.
出版信息
Curr Protoc Stem Cell Biol. 2014 Nov 3;31:1C.17.1-13. doi: 10.1002/9780470151808.sc01c17s31.
Abstract
This unit describes a cryopreservation procedure using an enzyme-free dissociation method to harvest cells and preserve cells in albumin-free chemically defined E8 medium for human pluripotent stem cells (hPSCs). The dissociation by EDTA/PBS produces small cell aggregates that allow high survival efficiency in passaging and cryopreservation. Cryopreservation in E8 medium eliminates serum and other animal products, and is suitable for dealing with the increasing demand for high-quality hPSCs in translational research. In combination with the special feature of EDTA/PBS dissociation, the protocols in this unit allow for efficient cryopreservation in a more time-saving manner.
摘要
本单元介绍了一种使用无酶解离方法收获细胞，并将人多能干细胞（hPSCs）保存在无白蛋白化学限定E8培养基中的冷冻保存程序。用EDTA/PBS进行解离会产生小细胞聚集体，从而在传代和冷冻保存中具有较高的存活效率。在E8培养基中进行冷冻保存可消除血清和其他动物产品，适用于应对转化研究中对高质量hPSCs日益增长的需求。结合EDTA/PBS解离的特点，本单元中的方案能够以更节省时间的方式进行高效冷冻保存。
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