Borawska J, Darewicz M, Vegarud G E, Iwaniak A, Minkiewicz P
Department of Food Biochemistry, Faculty of Food Science, University of Warmia and Mazury in Olsztyn, Cieszyński 1, 10-726 Olsztyn, Poland.
Food Funct. 2015 Jan;6(1):211-8. doi: 10.1039/c4fo00621f. Epub 2014 Nov 4.
In the digestive tract of humans, bioactive peptides, i.e. protein fragments impacting the physiological activity of the body, may be released during the digestion of food proteins, including those of fish. The aim of the study was to establish the method of human ex vivo digestion of carp muscle tissue and evaluate the angiotensin I-converting enzyme inhibitory and antioxidant activities of hydrolysates obtained after digestion. It was found that the hydrolysates of carp muscle tissue obtained with the three-stage method of simulated ex vivo digestion showed ACE inhibitory as well as antioxidative activities. It was demonstrated that the degree of hydrolysis depended on the duration of individual stages and the degree of comminution of the examined material. Although the applied gastric juices initiated the process of hydrolysis of carp muscle tissue, the duodenal juices caused a rapid increase in the amount of hydrolysed polypeptide bonds. The antihypertensive and antioxidative activities of the hydrolysates of carp muscle tissue increased together with progressive protein degradation. However, the high degree of protein hydrolysis does not favour an increase in the activity of free radical scavenging. The presented results are an example of the first preliminary screening of the potential health-promoting biological activity of carp muscle tissue in an ex vivo study.
在人类消化道中，生物活性肽，即影响身体生理活动的蛋白质片段，可能在包括鱼类蛋白质在内的食物蛋白质消化过程中释放出来。本研究的目的是建立鲤鱼肌肉组织的人体体外消化方法，并评估消化后获得的水解产物的血管紧张素I转换酶抑制活性和抗氧化活性。结果发现，采用模拟体外消化的三阶段方法获得的鲤鱼肌肉组织水解产物具有ACE抑制活性和抗氧化活性。结果表明，水解程度取决于各个阶段的持续时间和受试材料的粉碎程度。虽然所应用的胃液启动了鲤鱼肌肉组织的水解过程，但十二指肠液导致水解多肽键的数量迅速增加。鲤鱼肌肉组织水解产物的降压和抗氧化活性随着蛋白质的逐步降解而增加。然而，高度的蛋白质水解不利于自由基清除活性的增加。所呈现的结果是在体外研究中对鲤鱼肌肉组织潜在的促进健康生物活性进行首次初步筛选的一个例子。
