从组织培养细胞中分离网格蛋白包被小泡。
Isolation of clathrin-coated vesicles from tissue culture cells.
作者信息
Borner Georg H H, Fielding Andrew B
机构信息
Department of Proteomics and Signal Transduction, Max Plank Institute of Biochemistry, 82152 Martinsried, Germany;
Department of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool L69 3BX, United Kingdom.
出版信息
Cold Spring Harb Protoc. 2014 Nov 3;2014(11):1136-8. doi: 10.1101/pdb.top074435.
The study of clathrin-coated vesicles (CCVs) isolated from various organs has revealed the identities and important features of many of the factors involved in membrane trafficking. The development of isolation methods using cultured cell lines has made it possible to manipulate the source material before isolation to ask important questions about the roles of these factors and the pathways in which they are involved. We discuss here the advantages and limitations of the use of cultured cell lines for the isolation of CCVs.
对从各种器官分离出的网格蛋白包被小泡（CCV）的研究揭示了许多参与膜运输的因子的特性和重要特征。利用培养细胞系的分离方法的发展使得在分离前对原材料进行操控成为可能，从而能够探讨这些因子的作用以及它们所涉及的途径等重要问题。我们在此讨论使用培养细胞系分离CCV的优点和局限性。
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