Purification of Clathrin-Coated Vesicles from Adult Rat Brain.
作者信息
Giovedì Silvia
机构信息
Department of Experimental Medicine, University of Genova, Genoa, Italy.
出版信息
Methods Mol Biol. 2018;1847:1-11. doi: 10.1007/978-1-4939-8719-1_1.
PMID:30129005
Abstract
Here, we describe a purification protocol for isolating clathrin-coated vesicles (CCVs) from adult rat brain by using differential centrifugation coupled with Ficoll-sucrose and DO-sucrose density gradient centrifugation and an additional linear sucrose step gradient at the end to separate CCVs from contaminating membranes present in the crude microsomal fraction.
摘要
Zotero 插件