Yang Juan, Yang Jin, Gao Yan, Zhao Lingyu, Liu Liying, Qin Yannan, Wang Xiaofei, Song Tusheng, Huang Chen
Key Laboratory of Environment and Genes Related to Diseases of the Education Ministry, Department of Genetics and Molecular Biology, Medical School of Xi'an Jiaotong University, Xi'an, China.
Department of Medical Oncology, First Affiliated Hospital of Medical School of Xi'an Jiaotong University, Xi'an, China.
PLoS One. 2014 Nov 4;9(11):e111364. doi: 10.1371/journal.pone.0111364. eCollection 2014.
To investigate discriminating protein patterns and serum biomarkers between clear cell renal cell carcinoma (ccRCC) patients and healthy controls, as well as between paired pre- and post-operative ccRCC patients.
We used magnetic bead-based separation followed by matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) to identify patients with ccRCC. A total of 162 serum samples were analyzed in this study, among which there were 58 serum samples from ccRCC patients, 40 from additional paired pre- and post-operative ccRCC patients (n = 20), and 64 from healthy volunteers as healthy controls. ClinProTools software identified several distinct markers between ccRCC patients and healthy controls, as well as between pre- and post-operative patients.
Patients with ccRCC could be identified with a mean sensitivity of 88.38% and a mean specificity of 91.67%. Of 67 m/z peaks that differed among the ccRCC, healthy controls, pre- and post-operative ccRCC patients, 24 were significantly different (P<0.05). Three candidate peaks, which were upregulated in ccRCC group and showed a tendency to return to healthy control values after surgery, were identified as peptide regions of RNA-binding protein 6 (RBP6), tubulin beta chain (TUBB), and zinc finger protein 3 (ZFP3) with the m/z values of 1466.98, 1618.22, and 5905.23, respectively.
MB-MALDI-TOF-MS method could generate serum peptidome profiles of ccRCC, and provide a new approach to identify potential biomarkers for diagnosis as well as prognosis of this malignancy.
研究透明细胞肾细胞癌（ccRCC）患者与健康对照之间，以及ccRCC患者手术前后配对样本之间的蛋白质模式差异和血清生物标志物。
我们采用基于磁珠分离，随后进行基质辅助激光解吸电离（MALDI）飞行时间（TOF）质谱（MS）的方法来识别ccRCC患者。本研究共分析了162份血清样本，其中58份来自ccRCC患者，40份来自另外20对ccRCC患者手术前后的配对样本，64份来自健康志愿者作为健康对照。ClinProTools软件识别出了ccRCC患者与健康对照之间，以及手术前后患者之间的几种不同标志物。
ccRCC患者的识别平均灵敏度为88.38%，平均特异性为91.67%。在ccRCC患者、健康对照、手术前后的ccRCC患者之间存在差异的67个m/z峰中，有24个差异显著（P<0.05）。三个候选峰在ccRCC组中上调，术后有恢复到健康对照值的趋势，它们分别被鉴定为RNA结合蛋白6（RBP6）、微管蛋白β链（TUBB）和锌指蛋白3（ZFP3）的肽段区域，m/z值分别为1466.98、1618.22和5905.23。
MB-MALDI-TOF-MS方法能够生成ccRCC的血清肽组图谱，并为识别这种恶性肿瘤的诊断和预后潜在生物标志物提供一种新方法。
