Jessop D S, Eckland D J, Todd K, Lightman S L
Medical Unit, Charing Cross and Westminster Medical School, Westminster Hospital, London.
J Endocrinol. 1989 Jan;120(1):119-24. doi: 10.1677/joe.0.1200119.
We have detected significant amounts of corticotrophin-releasing factor-41 (CRF-41) in the rat hypothalamo-neurointermediate lobe system using a radioimmunoassay and reversed-phase high-performance liquid chromatography. Total amounts of CRF-41 in extracts of median eminence (ME), supraoptic nucleus (SON), paraventricular nucleus (PVN) and neurointermediate lobe (NIL) from control animals were 1076 +/- 132, 196 +/- 44, 22 +/- 7 and 147 +/- 50 fmol respectively (means +/- S.E.M., n = 6). In animals given 340 mmol NaCl/l instead of tap water to drink for 12 days, no significant changes occurred in the CRF-41 content of the ME, SON or PVN, but CRF-41 content increased more than twofold in the NIL (362 +/- 58 fmol). Plasma concentrations of CRF-41 and ACTH in control animals were 23 +/- 6 and 51 +/- 8 pmol/l respectively. After saline treatment no significant change in plasma CRF-41 was detected (20 +/- 8 pmol/l) but concentrations of circulating ACTH were decreased (15 +/- 2 pmol/l). The CRF-41 content of both the ME and the NIL was significantly depleted after intracerebroventricular injection of colchicine (414 +/- 81 and 34 +/- 7 fmol respectively). These data suggest that NIL CRF-41 is of hypothalamic origin and can be regulated by an osmotic stimulus.
我们使用放射免疫分析法和反相高效液相色谱法，在大鼠下丘脑 - 神经中间叶系统中检测到了大量的促肾上腺皮质激素释放因子 - 41（CRF - 41）。来自对照动物的正中隆起（ME）、视上核（SON）、室旁核（PVN）和神经中间叶（NIL）提取物中CRF - 41的总量分别为1076±132、196±44、22±7和147±50 fmol（平均值±标准误，n = 6）。给动物饮用340 mmol NaCl/l而非自来水12天，ME、SON或PVN中CRF - 41的含量没有显著变化，但NIL中CRF - 41的含量增加了两倍多（362±58 fmol）。对照动物血浆中CRF - 41和促肾上腺皮质激素（ACTH）的浓度分别为23±6和51±8 pmol/l。盐水处理后，未检测到血浆CRF - 41有显著变化（20±8 pmol/l），但循环ACTH的浓度降低了（15±2 pmol/l）。脑室内注射秋水仙碱后，ME和NIL中CRF - 41的含量均显著减少（分别为414±81和34±7 fmol）。这些数据表明，NIL中的CRF - 41起源于下丘脑，并且可以受到渗透刺激的调节。
