Taylor Sarah E, Wood Joanna P, Thomas Anne L, Jones George D D, Reid Helen J, Sharp Barry L
Centre for Analytical Science, Department of Chemistry, Loughborough University, Loughborough, LE11 3TU, UK.
Anal Bioanal Chem. 2014 Dec;406(30):8033-6. doi: 10.1007/s00216-014-8274-x. Epub 2014 Nov 6.
This note presents a comparison of the use of saliva versus leukocytes for the determination of Pt-DNA adducts obtained from patients undergoing platinum-based chemotherapy. Samples of both blood and saliva were taken pre- and post-treatment and were analysed via sector-field inductively coupled plasma mass spectrometry (SF-ICP-MS) to determine the level of Pt-DNA adducts formed. As expected, significant inter-patient variability was seen; however, a lack of correlation between the levels of adducts observed in saliva and blood samples was also observed (Pearson correlation coefficient r = -0.2598). A high yield of DNA was obtained from saliva samples, but significant difficulties were experienced in obtaining patient adherence to the saliva sampling procedure. In both leukocyte and saliva samples, not only was Pt from previous chemotherapy cycles detected, but the rapid appearance of Pt in the DNA was noted in both sample types 1 h after treatment.
本笔记介绍了在接受铂类化疗的患者中，使用唾液与白细胞来测定铂-DNA加合物的比较情况。在治疗前和治疗后采集了血液和唾液样本，并通过扇形场电感耦合等离子体质谱法（SF-ICP-MS）进行分析，以确定形成的铂-DNA加合物水平。正如预期的那样，观察到患者之间存在显著的变异性；然而，在唾液和血液样本中观察到的加合物水平之间也缺乏相关性（皮尔逊相关系数r = -0.2598）。从唾液样本中获得了高产率的DNA，但在让患者坚持唾液采样程序方面遇到了重大困难。在白细胞和唾液样本中，不仅检测到了先前化疗周期中的铂，而且在治疗后1小时，两种样本类型的DNA中都注意到铂的快速出现。
