Luo Yunjing, Li Shaohui, She Yuanbin, Zhong Rugang, Zhang Shufen, An Xuejing
College of Life Science and Bio-engineering, Beijing University of Technology, Beijing, 100124, China,
Bull Environ Contam Toxicol. 2015 Jan;94(1):112-7. doi: 10.1007/s00128-014-1411-6. Epub 2014 Nov 6.
A porphyrin/peroxynitrite biomimetic system was used to study the metabolism of indeno[1,2,3-cd]pyrene (IND) induced by peroxynitrite. The metabolites were identified using high-performance liquid chromatography coupled with electro-spray ionization tandem mass spectrometry as OH-IND, IND-quinone and 2NO2-IND. By stopping the reaction at different stages, we discovered that IND was first transformed to IND-quinone and 2NO2-IND, which were then transformed to OH-IND. Mutation assays including Ames tests and cell transformation experiments showed enhancement of the mutagenicity after the activation by the peroxynitrite/Fe(III)porphyrin system. The results also showed that 2NO2-IND and IND-quinone played key roles in the mutagenicity of PAHs after metabolic activation.
使用卟啉/过氧亚硝酸盐仿生系统研究过氧亚硝酸盐诱导的茚并[1,2,3-cd]芘（IND）的代谢。代谢产物通过高效液相色谱与电喷雾电离串联质谱联用鉴定为OH-IND、IND-醌和2NO2-IND。通过在不同阶段终止反应，我们发现IND首先转化为IND-醌和2NO2-IND，然后再转化为OH-IND。包括Ames试验和细胞转化实验在内的突变试验表明，过氧亚硝酸盐/铁（III）卟啉系统激活后诱变性增强。结果还表明，2NO2-IND和IND-醌在多环芳烃代谢激活后的诱变性中起关键作用。
