State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun 130022, P. R. China.
ACS Appl Mater Interfaces. 2014 Dec 10;6(23):20868-79. doi: 10.1021/am506983q. Epub 2014 Nov 18.
Detection of dysfunctional and apoptotic cells plays an important role in clinical diagnosis and therapy. To develop a portable and user-friendly platform for dysfunctional and aging cell detection, we present a facile method to construct 3D patterns on the surface of styrene-b-(ethylene-co-butylene)-b-styrene elastomer (SEBS) with poly(ethylene glycol) brushes. Normal red blood cells (RBCs) and lysed RBCs (dysfunctional cells) are used as model cells. The strategy is based on the fact that poly(ethylene glycol) brushes tend to interact with phosphatidylserine, which is in the inner leaflet of normal cell membranes but becomes exposed in abnormal or apoptotic cell membranes. We demonstrate that varied patterned surfaces can be obtained by selectively patterning atom transfer radical polymerization (ATRP) initiators on the SEBS surface via an aqueous-based method and growing PEG brushes through surface-initiated atom transfer radical polymerization. The relatively high initiator density and polymerization temperature facilitate formation of PEG brushes in high density, which gives brushes worm-like morphology and superhydrophilic property; the tendency of dysfunctional cells adhered on the patterned surfaces is completely different from well-defined arrays of normal cells on the patterned surfaces, providing a facile method to detect dysfunctional cells effectively. The PEG-patterned surfaces are also applicable to detect apoptotic HeLa cells. The simplicity and easy handling of the described technique shows the potential application in microdiagnostic devices.
检测功能失调和凋亡细胞在临床诊断和治疗中起着重要作用。为了开发用于功能失调和衰老细胞检测的便携式和用户友好型平台，我们提出了一种在苯乙烯-丁二烯-苯乙烯嵌段共聚物（SEBS）表面上构建 3D 图案的简便方法，该方法使用聚乙二醇（PEG）刷。正常红细胞（RBC）和裂解的 RBC（功能失调的细胞）被用作模型细胞。该策略基于以下事实：PEG 刷倾向于与磷脂酰丝氨酸相互作用，而磷脂酰丝氨酸位于正常细胞膜的内叶，但在异常或凋亡细胞膜中暴露。我们证明，通过在 SEBS 表面上通过基于水的方法选择性地图案化原子转移自由基聚合（ATRP）引发剂，并通过表面引发的原子转移自由基聚合来生长 PEG 刷，可以获得各种图案化表面。相对较高的引发剂密度和聚合温度有利于高密度的 PEG 刷的形成，从而赋予刷蠕虫状形态和超亲水性；功能失调细胞在图案化表面上的附着趋势与图案化表面上规则排列的正常细胞完全不同，提供了一种有效检测功能失调细胞的简便方法。PEG 图案化表面也适用于检测凋亡的 HeLa 细胞。所描述的技术的简单性和易于处理显示了在微诊断设备中的潜在应用。
