Acevedo Cristian A, Díaz-Calderón Paulo, Enrione Javier, Caneo María J, Palacios Camila F, Weinstein-Oppenheimer Caroline, Brown Donald I
Centro de Biotecnología, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso, Chile,
Bioprocess Biosyst Eng. 2015 Apr;38(4):777-85. doi: 10.1007/s00449-014-1319-x. Epub 2014 Nov 7.
Biomaterials based on crosslinked sponges of biopolymers have been extensively used as scaffolds to culture mammal cells. It is well known that single biopolymers show significant change over time due to a phenomenon called physical ageing. In this research, it was verified that scaffolds used for skin tissue engineering (based on gelatin, chitosan and hyaluronic acid) express an ageing-like phenomenon. Treatments based on ageing of scaffolds improve the behavior of skin-cells for tissue engineering purposes. Physical ageing of dry scaffolds was studied by differential scanning calorimetry and was modeled with ageing kinetic equations. In addition, the physical properties of wet scaffolds also changed with the ageing treatments. Scaffolds were aged up to 3 weeks, and then skin-cells (fibroblasts) were seeded on them. Results indicated that adhesion, migration, viability, proliferation and spreading of the skin-cells were affected by the scaffold ageing. The best performance was obtained with a 2-week aged scaffold (under cell culture conditions). The cell viability inside the scaffold was increased from 60% (scaffold without ageing treatment) to 80%. It is concluded that biopolymeric scaffolds can be modified by means of an ageing treatment, which changes the behavior of the cells seeded on them. The ageing treatment under cell culture conditions might become a bioprocess to improve the scaffolds used for tissue engineering and regenerative medicine.
基于生物聚合物交联海绵的生物材料已被广泛用作培养哺乳动物细胞的支架。众所周知，由于一种称为物理老化的现象，单一生物聚合物会随时间发生显著变化。在本研究中，已证实用于皮肤组织工程的支架（基于明胶、壳聚糖和透明质酸）表现出类似老化的现象。基于支架老化的处理改善了用于组织工程目的的皮肤细胞的行为。通过差示扫描量热法研究了干燥支架的物理老化，并使用老化动力学方程进行建模。此外，湿支架的物理性质也随老化处理而改变。将支架老化长达3周，然后在其上接种皮肤细胞（成纤维细胞）。结果表明，支架老化影响皮肤细胞的粘附、迁移、活力、增殖和铺展。在2周老化的支架上（在细胞培养条件下）获得了最佳性能。支架内的细胞活力从60%（未经老化处理的支架）提高到80%。得出的结论是，可以通过老化处理来修饰生物聚合物支架，这会改变接种在其上的细胞的行为。细胞培养条件下的老化处理可能会成为一种生物工艺，以改善用于组织工程和再生医学的支架。
