Xu Hui-qing, Gao Lu, Jiang Yun-shen, Tian Ying, Peng Jin, Xa Qi-quan, Chen Yu
College of Food Science and Engineering, Yangzhou University, Yangzhou, Jiangsu, P. R. China.
J Basic Microbiol. 2015 Apr;55(4):490-9. doi: 10.1002/jobm.201400540. Epub 2014 Nov 11.
Lactobacillus sakei is a heterofermentative species of lactic acid bacteria that is used in industrial meat fermentation. To investigate adaptation in a meat environment, whole-genome DNA microarrays were used to analyze the gene expression related to growth and survival of L. sakei strain La22 when grown in sarcoplasmic (S-) or myofibrillar (M-) protein-supplemented chemically defined medium (CDM). Differential expression was detected in 551 genes. Genes encoding enzymes involved in peptide hydrolysis were differentially upregulated in M-CDM or/and S-CDM, and only oppB and oppC, involved in the amino acid and peptide transport system, were upregulated. Most genes related to metabolism of peptides, amino acids and related molecules were over-expressed in M-CDM and S-CDM, except for glnA and metK. Expression of certain genes was according to the differential substrate environment. The expression of genes involved in the stress response was not induced by growth in M-CDM.
清酒乳杆菌是一种用于工业肉类发酵的异型发酵乳酸菌。为了研究其在肉类环境中的适应性，利用全基因组DNA微阵列分析了清酒乳杆菌La22菌株在添加肌浆蛋白（S-）或肌原纤维蛋白（M-）的化学限定培养基（CDM）中生长时与生长和存活相关的基因表达。检测到551个基因存在差异表达。在M-CDM或/和S-CDM中，编码参与肽水解的酶的基因差异上调，且仅参与氨基酸和肽转运系统的oppB和oppC上调。除谷氨酰胺合成酶基因（glnA）和甲硫氨酸腺苷转移酶基因（metK）外，大多数与肽、氨基酸及相关分子代谢相关的基因在M-CDM和S-CDM中均过度表达。某些基因的表达取决于不同的底物环境。在M-CDM中生长并未诱导参与应激反应的基因表达。
