Seidman M
Molecular Biology Department, Otsuka Pharmaceutical Co., Ltd., Rockville, MD 20850.
Mutat Res. 1989 Mar-May;220(2-3):55-60. doi: 10.1016/0165-1110(89)90010-9.
Shuttle-vector plasmids would appear to provide a powerful technology for studying mutagenesis in mammalian (including human) cells. Recently, as described in this and other papers in this volume, several shuttle-vector systems have been described and applied. The development of the first shuttle vectors for these purposes was hindered by two major problems. The first of these was the 'poison' sequence present in many pBR322 based vectors. The second was the problem of spontaneous mutagenesis associated with transfection of the plasmids into mammalian cells. Effective solutions for both problems have been devised, and it is now possible to experimentally address a variety of questions concerning mutagenesis and repair in mammalian cells.
穿梭载体质粒似乎为研究哺乳动物（包括人类）细胞中的诱变作用提供了一种强大的技术。最近，正如本卷中这篇论文及其他论文所描述的，已经报道并应用了几种穿梭载体系统。用于这些目的的首批穿梭载体的开发受到两个主要问题的阻碍。第一个问题是许多基于pBR322的载体中存在的“毒性”序列。第二个问题是与将质粒转染到哺乳动物细胞中相关的自发诱变问题。针对这两个问题都已设计出有效的解决方案，现在有可能通过实验解决有关哺乳动物细胞诱变和修复的各种问题。
