Gold Matthew, Marsolais David, Blanchet Marie-Renee
The Biomedical Research Centre, Department of Experimental Medicine, The University of British Columbia, Vancouver, BC, Canada.
Methods Mol Biol. 2015;1220:503-19. doi: 10.1007/978-1-4939-1568-2_31.
In the last 20 years, the development of murine models of allergic asthma has provided researchers with a means to explore the mechanisms of this T-helper type 2 (Th2)-driven inflammatory disease. While systemic sensitization and airway challenge with ovalbumin has been the most widely used model, recent emphasis has been placed on the development of models using more naturally occurring antigens. However, the diversity of models currently available makes it hard for investigators new to this field to choose to use the most effective and appropriate model to test their hypothesis. Here we describe three different mouse models of allergic asthma, including the classical ovalbumin model, a modified ovalbumin model that has been shown to be mast-cell dependent, as well as a house dust mite antigen-induced model. We also discuss briefly their characterization and differences, in the aim to facilitate the choice of the appropriate model when working on this intricate Th2 inflammatory disease.
在过去20年中，过敏性哮喘小鼠模型的发展为研究人员提供了一种手段，用以探索这种由2型辅助性T细胞（Th2）驱动的炎症性疾病的发病机制。虽然用卵清蛋白进行全身致敏和气道激发是最广泛使用的模型，但最近人们的重点已放在使用更天然存在的抗原开发模型上。然而，目前可用模型的多样性使得该领域的新研究人员难以选择使用最有效和合适的模型来检验他们的假设。在此，我们描述三种不同的过敏性哮喘小鼠模型，包括经典的卵清蛋白模型、已证明依赖肥大细胞的改良卵清蛋白模型，以及屋尘螨抗原诱导的模型。我们还简要讨论了它们的特征和差异，目的是在研究这种复杂的Th2炎症性疾病时便于选择合适的模型。
