Khalid Rabia, Zhang Yu Jing, Ali Safdar, Sui Xin Hua, Zhang Xiao Xia, Amara Ummay, Chen Wen Xin, Hayat Rifat
Department of Soil Science & Soil Water Conservation, PMAS-Arid Agriculture University, Rawalpindi, Pakistan,
Antonie Van Leeuwenhoek. 2015 Jan;107(1):281-90. doi: 10.1007/s10482-014-0326-x. Epub 2014 Nov 13.
A Gram-negative, white, non-motile, rod shaped bacterial strain BN-19(T) was isolated from a root nodule of groundnut (Arachis hypogaea) in Pakistan. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain BN-19(T) formed a subclade in the genus Rhizobium together with Rhizobium alkalisoli CCBAU 01393(T), Rhizobium vignae CCBAU 05176(T), Rhizobium huautlense SO2(T) and Rhizobium tarimense PL-41(T) with sequence similarities of 97.5, 97.3, 97.2 and 97.1 % respectively. Sequence analysis of housekeeping genes atpD, glnII and recA (with sequence similarities of ≤92 %) confirmed the unique position of BN-19(T) in the genus Rhizobium. DNA-DNA relatedness between the strain BN-19(T) and R. alkalisoli CCBAU 01393(T), R. vignae CCBAU 05176(T), R. huautlense SO2(T) and R. tarimense PL-41(T) were 20.6, 22.5, 15.9 and 20.5 % respectively, further confirming that BN-19(T) represents a novel species in the genus Rhizobium. The DNA G + C content was 60.1 mol%. The dominant fatty acids of strain BN-19(T) were C19:0 cyclo ω8c, summed feature 2 (C14:0 3OH and/or C16:1 iso I) and summed feature 8 (C18:1 ω7c). Some phenotypic features also differentiate the strain BN-19(T) from the related species. On the basis of these results, strain BN-19(T) is considered to represent a novel species in the genus Rhizobium, for which the name Rhizobium pakistanensis sp. nov. is proposed. The type strain is BN-19(T) (=LMG 27895(T) = CCBAU 101086(T)).
从巴基斯坦花生（Arachis hypogaea）根瘤中分离出一株革兰氏阴性、白色、无运动性、杆状的细菌菌株BN-19(T)。基于16S rRNA基因序列的系统发育分析表明，菌株BN-19(T)与碱土根瘤菌CCBAU 01393(T)、豇豆根瘤菌CCBAU 05176(T)、瓦特尔根瘤菌SO2(T)和塔里木根瘤菌PL-41(T)一起在根瘤菌属中形成一个亚分支，序列相似性分别为97.5%、97.3%、97.2%和97.1%。看家基因atpD、glnII和recA（序列相似性≤92%）的序列分析证实了BN-19(T)在根瘤菌属中的独特地位。菌株BN-19(T)与碱土根瘤菌CCBAU 01393(T)、豇豆根瘤菌CCBAU 05176(T)、瓦特尔根瘤菌SO2(T)和塔里木根瘤菌PL-41(T)之间的DNA-DNA相关性分别为20.6%、22.5%、15.9%和20.5%，进一步证实BN-19(T)代表根瘤菌属中的一个新物种。DNA G + C含量为60.1 mol%。菌株BN-19(T)的主要脂肪酸为C19:0环ω8c、特征性总和2（C14:0 3OH和/或C16:ω7c）。一些表型特征也使菌株BN-19(T)与相关物种区分开来。基于这些结果，菌株BN-19(T)被认为代表根瘤菌属中的一个新物种，为此提出名称为巴基斯坦根瘤菌（Rhizobium pakistanensis sp. nov.）。模式菌株为BN-19(T)（=LMG 27895(T)=CCBAU 101086(T)）。
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