State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
Int J Syst Evol Microbiol. 2009 Dec;59(Pt 12):3006-11. doi: 10.1099/ijs.0.007237-0. Epub 2009 Jul 30.
Three rhizobial strains (CCBAU 01393(T), CCBAU 01389 and CCBAU 03239) isolated from nodules of Caragana intermedia grown in saline-alkaline soils in the north of China had identical 16S rRNA genes that showed 99.7 and 99.5 % sequence similarities with those of Rhizobium huautlense SO2(T) and Rhizobium galegae USDA 4128(T), respectively. Phylogenies of the housekeeping genes atpD, recA and glnII confirmed their distinct position, differing from recognized Rhizobium species. SDS-PAGE of whole-cell soluble proteins and a series of phenotypic and physiological tests allowed us to differentiate the novel group from all closely related recognized Rhizobium species. The levels of DNA-DNA relatedness between strain CCBAU 01393(T) and R. huautlense SO2(T) and R. galegae USDA 4128(T) were 34.9 and 20.5 %, respectively. Therefore, we propose that strains CCBAU 01393(T), CCBAU 01389 and CCBAU 03239 represent a novel species, Rhizobium alkalisoli sp. nov., with strain CCBAU 01393(T) (=LMG 24763(T)=HAMBI 3051(T)) as the type strain. This strain could form effective nodules on Caragana microphylla, Phaseolus vulgaris and Vigna radiata.
三株根瘤菌（CCBAU 01393(T)、CCBAU 01389 和 CCBAU 03239）从中国北方盐碱性土壤中生长的中间锦鸡儿的根瘤中分离出来，它们的 16S rRNA 基因完全相同，与 Rhizobium huautlense SO2(T) 和 Rhizobium galegae USDA 4128(T) 的序列相似性分别为 99.7%和 99.5%。看家基因 atpD、recA 和 glnII 的系统发育证实了它们的独特地位，与公认的根瘤菌物种不同。全细胞可溶性蛋白的 SDS-PAGE 和一系列表型和生理测试使我们能够将新组与所有密切相关的公认根瘤菌物种区分开来。菌株 CCBAU 01393(T)与 R. huautlense SO2(T)和 R. galegae USDA 4128(T)之间的 DNA-DNA 同源性水平分别为 34.9%和 20.5%。因此，我们建议菌株 CCBAU 01393(T)、CCBAU 01389 和 CCBAU 03239 代表一个新物种，即耐碱根瘤菌（Rhizobium alkalisoli）sp. nov.，其中菌株 CCBAU 01393(T)（=LMG 24763(T)=HAMBI 3051(T)）为模式菌株。该菌株可在中间锦鸡儿、菜豆和豇豆上形成有效的根瘤。
