通过荧光漂白恢复技术（FRAP）和计算机模拟分析生物分子动力学。

Analysis of biomolecular dynamics by FRAP and computer simulation.

作者信息

Geverts Bart, van Royen Martin E, Houtsmuller Adriaan B

机构信息

Department of Pathology, Josephine Nefkens Institute, Erasmus Optical Imaging Centre, Erasmus MC, Postbus 2040, Rotterdam, 3000 CA, The Netherlands.

出版信息

Methods Mol Biol. 2015;1251:109-33. doi: 10.1007/978-1-4939-2080-8_7.

DOI:10.1007/978-1-4939-2080-8_7

PMID:25391797

Abstract

We present a Monte Carlo simulation environment for modelling complex biological molecular interaction networks and for the design, validation, and quantitative analysis of FRAP assays to study these. The program is straightforward in its implementation and can be instructed through an intuitive script language. The simulation tool fits very well in a systems biology research setting that aims to maintain an interactive cycle of experiment-driven modelling and model-driven experimentation: the model and the experiment are in the same simulation. The full program can be obtained by request to the authors.

摘要

我们提出了一种蒙特卡罗模拟环境，用于对复杂的生物分子相互作用网络进行建模，以及对用于研究这些网络的荧光漂白恢复（FRAP）分析进行设计、验证和定量分析。该程序在实现上很简单，可以通过直观的脚本语言进行指令操作。该模拟工具非常适合系统生物学研究环境，旨在维持实验驱动建模和模型驱动实验的交互循环：模型和实验在同一模拟中。完整程序可向作者索取获得。