聚乙二醇对糖萼内分子动力学的影响。

The influence of poly(ethylene glycol) on the molecular dynamics within the glycocalyx.

作者信息

Pratsch L, Herrmann A, Schwede I, Meyer H W

机构信息

Sektion Biologie, Humboldt-Universität, Berlin, G.D.R.

出版信息

Biochim Biophys Acta. 1989 Apr 14;980(2):146-54. doi: 10.1016/0005-2736(89)90393-3.

DOI:10.1016/0005-2736(89)90393-3

PMID:2539192

Abstract

Interaction of polymers with cell surfaces is a question of general interest for cell aggregation and fusion. The molecular dynamics within the surface coat of human erythrocytes as well as alterations of membrane protein arrangement (IMPs) in the presence of poly(ethylene glycol) (PEG) were investigated by EPR spin labeling techniques and freeze-fracture electron microscopy, respectively. AT PEG concentrations which induce aggregation of erythrocytes the surface coat and the protein arrangement is not disturbed by the polymer. This implicate an exclusion of the polymer from the cell surface.

摘要

聚合物与细胞表面的相互作用是细胞聚集和融合方面普遍感兴趣的问题。分别通过电子顺磁共振自旋标记技术和冷冻断裂电子显微镜研究了聚乙二醇（PEG）存在下人红细胞表面被膜内的分子动力学以及膜蛋白排列（IMPs）的变化。在诱导红细胞聚集的PEG浓度下，聚合物不会干扰表面被膜和蛋白质排列。这意味着聚合物被排除在细胞表面之外。