O'Byrne K T, Lunn S F, Fraser H M
MRC Reproductive Biology Unit, Centre for Reproductive Biology, Edinburgh, UK.
J Reprod Fertil. 1989 Mar;85(2):641-6. doi: 10.1530/jrf.0.0850641.
Six long-term ovariectomized adult marmoset monkeys were treated at 0 h with 35 micrograms oestradiol benzoate s.c. to induce an LH surge. They were also treated with detirelix (an LHRH antagonist) at 0 h, 12 h and 24 h (Exp. 1), or at 0 h and 24 h (Exp. 2) at a dose of 300 micrograms/kg s.c., or received the detirelix vehicle alone at 0 h, 12 h and 24 h (Exp. 3). All animals received the three treatments, with at least 4 weeks between experiments. Blood samples were collected at 0 h and at 6-12 h intervals for 72 h after oestradiol for the determination of plasma LH by bioassay. In control animals, oestrogen treatment resulted in a decline in plasma LH from 30.0 +/- 5.8 at 0 h to 12.8 +/- 2.6 ng/ml at 6 h (negative feedback), followed by a positive feedback surge, reaching a maximum of 148.0 +/- 34.6 ng/ml at 24 h. Values then declined to pretreatment levels by 56 h. In contrast, antagonist-treated animals showed complete abolition of the expected increase at 24 h, the low levels of the negative feedback phase being maintained for 36-72 h. These results show that hypothalamic LHRH release is essential during the oestrogen-induced LH surge, and that a direct oestrogen-induced component at the pituitary level is not expressed in the absence of LHRH in the marmoset.
六只成年长期卵巢切除的狨猴在0小时皮下注射35微克苯甲酸雌二醇以诱导促黄体生成素（LH）激增。它们还在0小时、12小时和24小时（实验1），或在0小时和24小时（实验2）接受地瑞林（一种促性腺激素释放激素拮抗剂）治疗，剂量为300微克/千克皮下注射，或者在0小时、12小时和24小时仅接受地瑞林赋形剂（实验3）。所有动物接受这三种处理，实验之间至少间隔4周。在注射雌二醇后0小时以及之后72小时每隔6 - 12小时采集血样，通过生物测定法测定血浆LH。在对照动物中，雌激素处理导致血浆LH从0小时的30.0±5.8下降到6小时的12.8±2.6纳克/毫升（负反馈），随后出现正反馈激增，在24小时达到最大值148.0±34.6纳克/毫升。然后在56小时时值降至预处理水平。相比之下，拮抗剂处理的动物在24小时时预期的增加完全消失，负反馈阶段的低水平维持36 - 72小时。这些结果表明，在雌激素诱导的LH激增过程中，下丘脑促性腺激素释放激素（LHRH）释放至关重要，并且在狨猴中，在没有LHRH的情况下，垂体水平上直接由雌激素诱导的成分未表现出来。
