水溶性共聚物作为潜在抗癌疫苗的碳水化合物抗原递送
Carbohydrate antigen delivery by water soluble copolymers as potential anti-cancer vaccines.
作者信息
Qin Qian, Yin Zhaojun, Bentley Philip, Huang Xuefei
机构信息
Department of Chemistry, Michigan State University, Chemistry Building, Room 426, 578 S. Shaw Lane, East Lansing, MI 48824, USA.
出版信息
Medchemcomm. 2014 Aug 1;5(8):1126-1129. doi: 10.1039/C4MD00103F.
Abstract
Tumor associated carbohydrate antigens (TACAs) are overexpressed on tumor cells, which renders them attractive targets for anti-cancer vaccines. To overcome the poor immunogenicity of TACAs, we designed a polymer platform for antigen presentation by co-delivering TACA and helper T (Th) cell epitope on the same chain. The block copolymer was synthesized by cyanoxyl-mediated free radical polymerization followed by conjugation with a TACA Tn antigen and a mouse Th-cell peptide epitope derived from polio virus (PV) to afford the vaccine construct. The glycopolymer vaccine elicited an anti-Tn immune response with significant titers of IgG antibodies, which recognized Tn-expressing tumor cells.
摘要
肿瘤相关碳水化合物抗原（TACAs）在肿瘤细胞上过度表达，这使其成为抗癌疫苗的有吸引力的靶点。为了克服TACAs免疫原性差的问题，我们设计了一种聚合物平台，通过在同一条链上共同递送TACA和辅助性T（Th）细胞表位来进行抗原呈递。通过氰氧基介导的自由基聚合反应合成嵌段共聚物，然后与TACA Tn抗原和源自脊髓灰质炎病毒（PV）的小鼠Th细胞肽表位偶联，得到疫苗构建体。该糖聚合物疫苗引发了具有显著滴度IgG抗体的抗Tn免疫反应，这些抗体能够识别表达Tn的肿瘤细胞。
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