Souza de Carvalho A F, Ramos S E, Gonzaga de Carvalho T S, Pomarico de Souza Y C, Zangeronimo M G, Pereira L J, Solis Murgas L D
Department of Veterinary Medicine, Federal University of Lavras, Lavras, Minas Gerais, Brazil.
Departament of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Minas Gerais, Brazil.
Cryo Letters. 2014 Sep-Oct;35(5):361-70.
Embryo cryopreservation has been used for the creation of genetic banks with diploid resources, and among different techniques, vitrification is considered as the most promising method.
The goal is to evaluate the major aspects of the existing vitrification techniques and to evaluate their efficacy in terms of embryo morphology.
Electronic searches in the PubMed and ScienceDirect databases were performed with the keyword combination: fish, embryo and vitrification. Pubmed retrieved 26 articles and Science Direct resulted in 464 articles. For this review, only studies that developed and tested vitrification protocols in fish embryos were included. Research regarding cryoprotectant toxicity and permeability were excluded. There were no restrictions on publication date or language. With these criteria, a total of ten articles were evaluated.
In these articles, the major aspects to be considered for the development of new vitrification protocols are: the cryoprotectants' toxicity, the embryos' development stage, the exposure to and the permeability of the cryoprotectants, vitrification devices and vitrification-warning cycle.
The survival were limited, however, the preservation of embryonic morphology after thawing indicates the possibility of preserving fish embryos via the vitrification technique.
胚胎冷冻保存已被用于创建具有二倍体资源的基因库，在不同技术中，玻璃化被认为是最有前景的方法。
目的是评估现有玻璃化技术的主要方面，并根据胚胎形态评估其效果。
在PubMed和ScienceDirect数据库中进行电子检索，关键词组合为：鱼类、胚胎和玻璃化。PubMed检索到26篇文章，ScienceDirect检索到464篇文章。本综述仅纳入了在鱼类胚胎中开发和测试玻璃化方案的研究。排除了关于冷冻保护剂毒性和渗透性的研究。对发表日期或语言没有限制。根据这些标准，共评估了10篇文章。
在这些文章中，开发新玻璃化方案时要考虑的主要方面有：冷冻保护剂的毒性、胚胎发育阶段、冷冻保护剂的暴露和渗透性、玻璃化装置以及玻璃化预警周期。
存活率有限，然而，解冻后胚胎形态的保存表明通过玻璃化技术保存鱼类胚胎是有可能的。
