Fischer S
Medizinische Klinik Innenstadt der Universität, München, F.R.G.
J Intern Med Suppl. 1989;731:21-4. doi: 10.1111/j.1365-2796.1989.tb01431.x.
Growing interest in nutritional intervention with n-3 polyunsaturated fatty acids demands reliable analyses of these fatty acids and their corresponding eicosanoids in vivo or ex vivo. n-3 polyunsaturated fatty acids are preferably assayed by capillary gas-liquid chromatography after extraction of the lipid classes, their separation by chromatographic methods and conversion of the fatty acids to methylesters. n-3 eicosanoids have to be separated from their arachidonic acid analogues by HPLC or by gas-liquid chromatography and are then quantified by UV-spectroscopy or mass-spectrometry. Structure elucidation has to be performed by chemical reactions and by mass spectrometry. Antibodies for radioimmunoassays against n-3 eicosanoids are not yet available.
对n-3多不饱和脂肪酸营养干预的兴趣日益浓厚，这就需要对这些脂肪酸及其相应的类二十烷酸进行可靠的体内或体外分析。n-3多不饱和脂肪酸最好在提取脂质类别后，通过毛细管气液色谱法进行测定，通过色谱方法将它们分离，并将脂肪酸转化为甲酯。n-3类二十烷酸必须通过高效液相色谱法或气液色谱法与花生四烯酸类似物分离，然后通过紫外光谱法或质谱法进行定量。结构解析必须通过化学反应和质谱法进行。针对n-3类二十烷酸的放射免疫分析抗体尚未获得。
