Atangana Etienne N, Homburg David, Vajkoczy Peter, Schneider Ulf C
Department of Neurosurgery, Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany.
Acta Neurochir (Wien). 2015 Jan;157(1):37-42. doi: 10.1007/s00701-014-2276-5. Epub 2014 Nov 16.
Brain atrophy after subarachnoid hemorrhage (SAH) has been detected in humans and might serve as a functional read-out parameter for neuropsychological deficits. To determine whether serial magnetic resonance imaging (MRI) can provide information on brain atrophy in animals as well, mice that had undergone experimental SAH were scanned repeatedly after the bleeding.
Using a 7-T rodent MRI, six mice were evaluated for total hemispheric, cerebrospinal fluid (CSF) and hippocampal volumes on days 1, 2, 4, 21, 28, 42 and 60 after experimental SAH or sham operation, respectively.
Repeated MRI scanning demonstrated a very high reproducibility with minimum standard deviation. Nevertheless, no significant differences were found between the two groups concerning hemispherical volumes or hippocampal volumes. A transient but significant increase in CSF volume was detected on days 2 and 60 after SAH. Compared with the existing method, no MRI data on brain atrophy in mice after experimental SAH have been published.
Repeated brain MRI in mice after experimental SAH did not provide additional information on brain atrophy. Our data suggest that this is not due to a lack of sensitivity of the method. Despite all promising details about MRI, our results should initiate careful consideration (additional sequences/other questions) before its further use in this certain area, especially since it is expensive and associated with demanding logistics.
蛛网膜下腔出血（SAH）后大脑萎缩在人类中已被检测到，并且可能作为神经心理缺陷的一个功能性读出参数。为了确定连续磁共振成像（MRI）是否也能提供动物大脑萎缩的信息，对经历过实验性SAH的小鼠在出血后进行了反复扫描。
使用一台7-T啮齿动物MRI，分别在实验性SAH或假手术后的第1、2、4、21、28、42和60天，对6只小鼠的全脑半球、脑脊液（CSF）和海马体积进行评估。
反复MRI扫描显示出非常高的可重复性，标准差最小。然而，两组在半球体积或海马体积方面未发现显著差异。在SAH后的第2天和第60天检测到脑脊液体积短暂但显著增加。与现有方法相比，尚无关于实验性SAH后小鼠大脑萎缩的MRI数据发表。
实验性SAH后对小鼠进行反复脑部MRI检查并未提供关于大脑萎缩的额外信息。我们的数据表明这并非由于该方法缺乏敏感性。尽管MRI有诸多有前景的细节，但我们的结果应促使在该特定领域进一步使用MRI之前进行仔细考虑（额外序列/其他问题），尤其是因为它昂贵且涉及复杂的后勤工作。
