Department of Neurosurgery, Medical Faculty, Heinrich Heine University, Moorenstraße 5, 40225, Düsseldorf, Germany.
Institute for Neurophysiology, Medical Faculty, University of Cologne, Robert-Koch-Str. 39, 50931, Köln, Germany.
Transl Stroke Res. 2017 Jun;8(3):206-219. doi: 10.1007/s12975-016-0513-3. Epub 2017 Jan 30.
Animal models are established to display the pathophysiological changes following subarachnoid hemorrhage (SAH). The aim of the present study was to determine case fatality in mouse delayed cerebral ischemia (DCI) models, to compare mortality in mouse DCI models to case fatality in human SAH patients, and to identify factors influencing mouse mortality. A systematic search of the PubMed database was performed to identify all studies that assessed mouse DCI models. Mortality rates and predictor variables were extracted and compared to the human case fatality after SAH as previously reported. Predictors for mouse mortality were identified through multivariate analysis. Forty-eight studies were included in the quantitative analysis. The mean overall mortality rate was 21% in mouse DCI models. However, the time period between induction of SAH and evaluation of mortality rates is a significant variable influencing the mortality rate in mouse SAH models. The experimental SAH model was the only significant predictor for mouse mortality after 48 h. In contrast, neither the genetic background nor the anesthetic changed the case fatality rate. Mouse mortality at 24, 48, and 72 h after experimental SAH in DCI models was significantly lower than human case fatality following aneurysmal SAH. The mean overall mortality rate in mouse DCI models is significantly lower than human case fatality following aneurysmal SAH. However, time between SAH induction and evaluation is a significant variable influencing the mortality rate in mouse SAH models. Further analyses will be required to establish whether and to which extent different DCI models affect mortality and reflect human pathophysiology.
动物模型被建立用于显示蛛网膜下腔出血（SAH）后病理生理变化。本研究的目的是确定小鼠迟发性脑缺血（DCI）模型中的病死率，比较小鼠 DCI 模型中的死亡率与人类 SAH 患者的病死率，并确定影响小鼠死亡率的因素。通过系统搜索 PubMed 数据库，确定了所有评估小鼠 DCI 模型的研究。提取死亡率和预测变量，并与之前报道的人类 SAH 后病死率进行比较。通过多变量分析确定了影响小鼠死亡率的预测因素。48 项研究被纳入定量分析。在小鼠 DCI 模型中，总的死亡率平均值为 21%。然而，SAH 诱导与死亡率评估之间的时间间隔是影响小鼠 SAH 模型死亡率的重要变量。实验性 SAH 模型是 48 小时后影响小鼠死亡率的唯一显著预测因素。相比之下，遗传背景或麻醉均未改变病死率。在 DCI 模型中，实验性 SAH 后 24、48 和 72 小时的小鼠死亡率明显低于动脉瘤性 SAH 后人类的病死率。在 DCI 模型中，与动脉瘤性 SAH 后人类病死率相比，小鼠总的死亡率平均值明显较低。然而，SAH 诱导与评估之间的时间间隔是影响小鼠 SAH 模型死亡率的重要变量。需要进一步分析以确定不同的 DCI 模型是否以及在何种程度上影响死亡率并反映人类病理生理学。
