Liu Yu-Lin, Sun Ya-Nan
Department of Otolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China.
Mol Biol Rep. 2014 Dec;41(12):7743-7. doi: 10.1007/s11033-014-3634-3. Epub 2014 Nov 16.
Testes-specific protease 50 is a newly reported threonine enzyme. It has similar amino acid sequences and enzymatic structures to some other serine proteases. It is proposed as a laryngocarcinoma-related gene in human beings. The physiological mechanism by which TSP50 exerts its promoting effects in laryngocarcinoma is not yet fully understood. The study investigated the function of TSP50 by suppressing its expression in the HEp2 cell line using a TSP50-specific short hairpin RNA (shRNA). Western bloting and real-time-PCR were used to detect the levels of TSP50. By using MTT, Wound healing, flow cytometric and tumorigenesis assays, the study tested the TSP50 role in human laryngocarcinoma cell growth and apoptosis. The results demonstrated that TSP50 knockdown could inhibit HEp2 cell proliferation and induce apoptosis in vitro in a NF-κB-mediated pathway. The tumorigenicity of TSP50 shRNA-expressing cells were decreased after inoculating into nude mice. The present results provide a new understanding of the TSP50 gene in the progression of laryngocarcinoma and put up a novel therapeutic target for treating this cancer.
睾丸特异性蛋白酶50是一种新报道的苏氨酸酶。它与其他一些丝氨酸蛋白酶具有相似的氨基酸序列和酶结构。它被认为是人类喉癌相关基因。TSP50在喉癌中发挥促进作用的生理机制尚未完全明确。该研究通过使用TSP50特异性短发夹RNA（shRNA）抑制HEp2细胞系中TSP50的表达来研究其功能。采用蛋白质免疫印迹法和实时定量PCR检测TSP50的水平。通过MTT法、伤口愈合实验、流式细胞术和肿瘤发生实验，该研究检测了TSP50在人喉癌细胞生长和凋亡中的作用。结果表明，敲低TSP50可在体外通过NF-κB介导的途径抑制HEp2细胞增殖并诱导凋亡。将表达TSP50 shRNA的细胞接种到裸鼠体内后，其致瘤性降低。目前的研究结果为TSP50基因在喉癌进展中的作用提供了新的认识，并为治疗这种癌症提出了新的治疗靶点。
