Starý Petr, Kavallieratos Nickolas G, Petrović Andjeljko, Žikić Vladimir, Rakhshani Ehsan, Tomanović Snežana, Tomanović Željko, Havelka Jan
Laboratory of Aphidology, Institute of Entomology, Biology Centre, Academy of Sciences of the Czech Republic, Branišovská 31, 37005 Česke Budějovice, Czech Republic.
Laboratory of Agricultural Entomology, Department of Entomology and Agricultural Zoology, Benaki Phytopathological Institute, 8 Stefanou Delta st. 14561, Kifissia, Attica, Greece.
J Insect Sci. 2014 Jan 1;14:171. doi: 10.1093/jisesa/ieu033. Print 2014.
This study provides evidence on integrating the morphological, field, and laboratory data, and application of the cytochrome oxidase subunit I (COI) barcoding gene to the three asexual or sexual Lysiphlebus spp., i.e., Lysiphlebus cardui (Marshall), Lysiphlebus confusus Tremblay and Eady and Lysiphlebus fabarum (Marshall) (Hymenoptera: Braconidae: Aphidiinae). New aphid- invasive plant association, Aphis fabae Scopoli (Hemipreta: Aphididae) on Impatiens glandulifera Royle, has been used in the same model area in the Czech Republic under the same sampling and rearing method for several consecutive years and throughout the season. For molecular identification of these three species, we used DNA sequences of the barcoding region of the mitochondrial COI gene. Although our results confirmed ecological and morphological differences among L. cardui, L. confusus, and L. fabarum, genetic analysis on the basis of COI mitochondrial barcoding gene does not support species status of the mentioned Lysiphlebus taxa. The level of morphological differentiation in these Lysiphlebus Förster species is in accordance with the usual species variability within subfamily Aphidiinae. However, it should be examined how appearance of asexual lineages affects the morphological or genetical variability.
本研究提供了关于整合形态学、野外和实验室数据的证据，并将细胞色素氧化酶亚基I（COI）条形码基因应用于三种无性或有性的蚜茧蜂属物种，即卡氏蚜茧蜂（马歇尔）、混淆蚜茧蜂特伦布莱和伊迪以及豆蚜茧蜂（马歇尔）（膜翅目：茧蜂科：蚜茧蜂亚科）。新的蚜虫-入侵植物关联，即蚕豆蚜（半翅目：蚜科）与凤仙花（凤仙花科），已在捷克共和国的同一模型区域内，在相同的采样和饲养方法下，连续数年且在整个季节中使用。为了对这三个物种进行分子鉴定，我们使用了线粒体COI基因条形码区域的DNA序列。尽管我们的结果证实了卡氏蚜茧蜂、混淆蚜茧蜂和豆蚜茧蜂之间的生态和形态差异，但基于COI线粒体条形码基因的遗传分析并不支持上述蚜茧蜂分类单元的物种地位。这些蚜茧蜂属物种的形态分化水平与蚜茧蜂亚科内通常的物种变异性一致。然而，应该研究无性谱系的出现如何影响形态或遗传变异性。
