针对多聚体伤寒沙门氏菌重组溶血素E抗原的多亚型抗体反应。

Multi-isotype antibody responses against the multimeric Salmonella Typhi recombinant hemolysin E antigen.

作者信息

Ong Eugene Boon Beng, Ignatius Joshua, Anthony Amy Amilda, Aziah Ismail, Ismail Asma, Lim Theam Soon

机构信息

Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800, Minden, Malaysia.

出版信息

Microbiol Immunol. 2015 Jan;59(1):43-7. doi: 10.1111/1348-0421.12211.

DOI:10.1111/1348-0421.12211

PMID:25399538

Abstract

The detection and measurement of different antibody isotypes in the serum provide valuable indicators of the different stages of typhoid infection. Here, the ability of S. Typhi recombinant hemolysin E (HlyE) to detect multi-isotype antibody responses in sera of patients with typhoid and paratyphoid A was investigated using an indirect antibody immunoassay. Nanogram amounts of HlyE were found to be sufficient for detection of IgG and IgA isotypes and, in a study of individuals' sera (n = 100), the immunoassay was able to distinguish between typhoid and non-typhoid sera. The overall sensitivity, specificity and efficiency of the ELISA were 70% (39/56), 100% (44/44) and 83% respectively.

摘要

血清中不同抗体亚型的检测和测量为伤寒感染的不同阶段提供了有价值的指标。在此，利用间接抗体免疫测定法研究了伤寒杆菌重组溶血素E（HlyE）检测伤寒和甲型副伤寒患者血清中多亚型抗体反应的能力。发现纳克量的HlyE足以检测IgG和IgA亚型，并且在一项针对个体血清（n = 100）的研究中，该免疫测定法能够区分伤寒血清和非伤寒血清。ELISA的总体敏感性、特异性和效率分别为70%（39/56）、100%（44/44）和83%。