Wang Feng, Zhang Guangyuan, Zhou Yang, Gui Dingkun, Li Junhui, Xing Tao, Wang Niansong
Department of Nephrology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China.
Department of Urology, Shanghai Jiao Tong University Affiliated First People's Hospital, 100 Haining Road, Shanghai 200080, China.
Oxid Med Cell Longev. 2014;2014:203458. doi: 10.1155/2014/203458. Epub 2014 Oct 21.
Magnolin is the major active ingredient of the herb Magnolia fargesii which has anti-inflammatory and antioxidative effects. Oxidative stress and apoptosis are involved in the pathogenesis of contrast-induced nephropathy (CIN). We hypothesize that Magnolin could protect against CIN through antioxidative and antiapoptotic properties.
To test whether Magnolin could attenuate CIN, oxidative stress and apoptosis, in vivo and in vitro, we utilized a rat model of ioversol-induced CIN and a cell model of oxidative stress in which HK2 cells were treated with H2O2. Rats were assigned to 4 groups (n = 6 per group): control group, ioversol group (ioversol-induced CIN), vehicle group (CIN rats pretreated with vehicle), and Magnolin group (CIN rats pretreated with 1 mg/kg Magnolin).
The results showed that magnolin ameliorated the renal tubular necrosis, apoptosis, and the deterioration of renal function (P < 0.05). Furthermore, Magnolin reduced the renal oxidative stress, suppressed caspase-3 activity, and increased Bcl-2 expression in vivo and in vitro.
Magnolin might protect CIN in rats through antioxidation and antiapoptosis.
木兰脂素是凹叶厚朴的主要活性成分，具有抗炎和抗氧化作用。氧化应激和细胞凋亡参与了造影剂肾病（CIN）的发病机制。我们推测木兰脂素可通过抗氧化和抗凋亡特性预防CIN。
为了在体内和体外测试木兰脂素是否能减轻CIN、氧化应激和细胞凋亡，我们使用了碘海醇诱导的CIN大鼠模型和HK2细胞经H2O2处理的氧化应激细胞模型。将大鼠分为4组（每组n = 6）：对照组、碘海醇组（碘海醇诱导的CIN组）、溶剂组（用溶剂预处理的CIN大鼠）和木兰脂素组（用1mg/kg木兰脂素预处理的CIN大鼠）。
结果显示，木兰脂素改善了肾小管坏死、细胞凋亡以及肾功能恶化（P < 0.05）。此外，木兰脂素在体内和体外均降低了肾脏氧化应激，抑制了caspase-3活性，并增加了Bcl-2表达。
木兰脂素可能通过抗氧化和抗凋亡作用保护大鼠免受CIN。
Zotero 插件
