Mortensen Leiv M, Gislerød Hans R
Department of Plant Science, The University of Life Sciences, Ås NO-1432, Norway.
AMB Express. 2014 Jun 18;4:49. doi: 10.1186/s13568-014-0049-4. eCollection 2014.
Flue gases from a power plant based on waste combustion were tested as a carbon dioxide (CO2) source for growing Chlamydomonas reinhardtii. To achieve recognition as an environmentally friendly hydrogen production method, waste gases should be used to grow this hydrogen-producing microalgae. The algae were grown in undiluted flue gas containing 11.4±0.2% CO2 by volume, in diluted flue gas containing 6.7±0.1% or 2.5±0.0% CO2, and in pure liquid CO2 at a concentration of 2.7±0.2%. The NOx concentration was 45±16 mg m(-3), the SO2 concentration was 36±19 mg m(-3), the HCl concentration 4.1±1.0 mg m(-3) and the O2 concentration 7.9±0.2% in the undiluted flue gas. Undiluted flue gas reduced the dry weight production by around 20-25% when grown at a photon flux density (PFD) of 300 μmol m(-2) s(-1) artificial light and at 24 or 33°C, compared with the other treatments. A less negative effect was found at the highest flue gas concentration when the algae were grown at 75 μmol m(-2) s(-1) PFD. Growing the algae outdoors at a day length of 12.5 h and a temperature of around 24°C, the dry weight production was higher (about 15%) in the 2.6% CO2 flue gas treatment compared with all other treatments. Reducing the light level by 30% through shading did not affect the dry weight production. Calculated on aerial basis the productivity reached approximately 70 g m(-2) day(-1) in the 300 μmol m(-2) s(-1) PFD treatment (corresponding to 25 mol m(-2) day(-1)) and approximately 17 g m(-2) day(-1) in the 75μmol m(-2) s(-1) PFD treatment (corresponding to 6.5 mol m(-2) day(-1)). The outdoor production reached around 14 g m(-2) day(-1). It was concluded that the negative effect of the undiluted flue gas was attributable to the high CO2 concentration and not to the other pollutants.
对基于垃圾焚烧的发电厂产生的烟道气进行了测试，以确定其作为莱茵衣藻生长所需二氧化碳（CO₂）来源的可行性。为了被认可为一种环境友好型制氢方法，应使用废气来培养这种产氢微藻。藻类分别在体积分数为11.4±0.2% CO₂的未稀释烟道气、体积分数为6.7±0.1%或2.5±0.0% CO₂的稀释烟道气以及浓度为2.7±0.2%的纯液态CO₂中培养。未稀释烟道气中的氮氧化物（NOₓ）浓度为45±16 mg m⁻³，二氧化硫（SO₂）浓度为36±19 mg m⁻³，氯化氢（HCl）浓度为4.1±1.0 mg m⁻³，氧气（O₂）浓度为7.9±0.2%。与其他处理方式相比，当在300 μmol m⁻² s⁻¹的人工光照光子通量密度（PFD）以及24或33°C条件下培养时，未稀释烟道气使干重产量降低了约20 - 25%。当藻类在75 μmol m⁻² s⁻¹的PFD下培养时，在最高烟道气浓度下发现的负面影响较小。在日长为12.5小时、温度约为24°C的室外环境中培养藻类时，与所有其他处理方式相比，2.6% CO₂烟道气处理下的干重产量更高（约高15%）。通过遮光将光照强度降低30%并不影响干重产量。以单位面积计算，在300 μmol m⁻² s⁻¹的PFD处理下（相当于25 mol m⁻² day⁻¹）生产力约达到70 g m⁻² day⁻¹，在75 μmol m⁻² s⁻¹的PFD处理下（相当于6.5 mol m⁻² day⁻¹）约为17 g m⁻² day⁻¹。室外产量约为14 g m⁻² day⁻¹。研究得出结论，未稀释烟道气的负面影响归因于高CO₂浓度，而非其他污染物。
