Miao Zhijing, Chen Min, Wu Hong, Ding Hongjuan, Shi Zhonghua
State Key Laboratory of Reproductive Medicine, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210004, China.
Cell Physiol Biochem. 2014;34(5):1701-10. doi: 10.1159/000366371. Epub 2014 Nov 7.
Fetal growth restriction (FGR) is the main cause of intrauterine fetal death and the second leading cause of death in the neonatal period. A large body of evidence suggests that FGR may be associated with the placenta, although its etiology and pathogenesis remain to be fully elucidated.
To better understand the molecular mechanisms underlying the pathological development of the placenta in FGR, we used tandem mass tags (TMTs) to construct a large-scale comparative proteomic profile of human placentas from normal and FGR pregnancies. A total of 1,198 kinds of proteins were identified in the control and FGR placentas, of which 95 were differentially expressed between two groups. Ingenuity Pathway Analysis (IPA) was used to organize these differentially expressed proteins into networks of interacting proteins and to identify the modules of functionally related proteins. Western blotting was used to verify the expression patterns of several randomly selected proteins.
The placentas of women with FGR displayed significant proteome differences compared with normal pregnancy. The results indicate that a variety of mechanisms and proteins may contribute to the development of FGR. Further studies and validations are required to elucidate the exact roles of these proteins in FGR pathogenesis.
胎儿生长受限（FGR）是宫内胎儿死亡的主要原因，也是新生儿期死亡的第二大原因。大量证据表明，FGR可能与胎盘有关，尽管其病因和发病机制仍有待充分阐明。
为了更好地理解FGR中胎盘病理发育的分子机制，我们使用串联质谱标签（TMT）构建了正常妊娠和FGR妊娠的人胎盘大规模比较蛋白质组图谱。在对照胎盘和FGR胎盘中共鉴定出1198种蛋白质，其中两组间有95种蛋白质差异表达。利用 Ingenuity 通路分析（IPA）将这些差异表达的蛋白质组织成相互作用蛋白质网络，并识别功能相关蛋白质模块。采用蛋白质免疫印迹法验证了几种随机选择的蛋白质的表达模式。
与正常妊娠相比，FGR女性的胎盘显示出显著的蛋白质组差异。结果表明，多种机制和蛋白质可能参与了FGR的发生发展。需要进一步的研究和验证来阐明这些蛋白质在FGR发病机制中的具体作用。
