Masson M, Albouz S, Boutry J M, Spezzatti B, Castagna M, Baumann N
Unité de Neurobiologie Cellulaire Moléculaire et Clinique (INSERM U. 134), Paris, France.
J Neurochem. 1989 May;52(5):1645-7. doi: 10.1111/j.1471-4159.1989.tb09221.x.
N-(6-Aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7) is known to be a potent calmodulin antagonist and inhibitor of calmodulin-dependent protein kinases. W-7 and 1-(5-isoquinolinyl-sulfonyl)-2-methylpiperazine (H-7) are inhibitors of protein kinase C and cyclic nucleotide-dependent protein kinases. In C6 glioma cells, W-7 and not H-7 inhibited dose-dependently acid sphingomyelinase, a result indicating the modulation of this lysosomal enzyme by a calmodulin-dependent system. Other lysosomal enzymes, such as beta-glucosidase, alpha-galactosidase, and arylsulfatase A, were unaffected by W-7 and H-7, a finding indicating a selective effect of W-7 on sphingomyelinase.
N-(6-氨基己基)-5-氯-1-萘磺酰胺（W-7）是一种强效的钙调蛋白拮抗剂及钙调蛋白依赖性蛋白激酶抑制剂。W-7和1-(5-异喹啉磺酰基)-2-甲基哌嗪（H-7）是蛋白激酶C和环核苷酸依赖性蛋白激酶的抑制剂。在C6胶质瘤细胞中，W-7而非H-7能剂量依赖性地抑制酸性鞘磷脂酶，这一结果表明该溶酶体酶受钙调蛋白依赖性系统的调节。其他溶酶体酶，如β-葡萄糖苷酶、α-半乳糖苷酶和芳基硫酸酯酶A，不受W-7和H-7的影响，这一发现表明W-7对鞘磷脂酶具有选择性作用。
