Dong Ping, Fu Xiao, Wang Xiang, Wang Wen-mei, Cao Wen-ming, Zhang Wei-yun
State Key Laboratory of Analytical Chemistry for Life Science and Jiangsu Key Laboratory of Molecular Medicine, Nanjing University Medical School, 22 Hankou Road, Nanjing, 210093, China.
Cell Biochem Biophys. 2015 Mar;71(2):1207-13. doi: 10.1007/s12013-014-0330-9.
The aim of this study is to investigate protective effects of sesaminol on the human bronchial epithelial (BEAS-2B) cell line against oxidative damage of cigarette smoke extract (CSE). BEAS-2B cells were pre-incubated with sesaminol for 12 h and then treated with various concentrations of CSE for 24 h. After that proliferation ability, levels of reactive oxygen species (ROS) and lactate dehydrogenase (LDH), cell apoptosis, activities of catalase (CAT) and superoxide dismutase (SOD), and mRNA levels of IL-8 and IL-6 were measured. The results showed that sesaminol significantly improved BEAS-2B cell viability, reduced the production of ROS and LDH of cells, inhibited cell apoptosis and increased CAT and SOD activities in CSE-treated cells. Sesaminol also inhibited the expression of IL-8 and IL-6 mRNA following CSE exposure. In conclusion, sesaminol may protect BEAS-2B cells against CSE-induced oxidative damage.
本研究旨在探讨芝麻酚对人支气管上皮（BEAS-2B）细胞系抵御香烟烟雾提取物（CSE）氧化损伤的保护作用。将BEAS-2B细胞与芝麻酚预孵育12小时，然后用不同浓度的CSE处理24小时。之后检测细胞增殖能力、活性氧（ROS）和乳酸脱氢酶（LDH）水平、细胞凋亡情况、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性以及IL-8和IL-6的mRNA水平。结果表明，芝麻酚显著提高了CSE处理细胞中BEAS-2B细胞的活力，降低了细胞ROS和LDH的产生，抑制了细胞凋亡，并增加了CAT和SOD活性。芝麻酚还抑制了CSE暴露后IL-8和IL-6 mRNA的表达。总之，芝麻酚可能保护BEAS-2B细胞免受CSE诱导的氧化损伤。
