Divsalar Adeleh, Razmi Mahdieh, Saboury Ali Akbar, Mansouri-Torshizi Hassan, Ahmad Faizan
Department of Cell & Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
Cell Biochem Biophys. 2015 Apr;71(3):1415-24. doi: 10.1007/s12013-014-0364-z.
In this study, the interaction of β-casein (β-CN) with a new synthesized Pt(II) complex (bipyridin morpholin dithiocarbamate Pt(II) nitrate), as an anticancer compound, was studied. This study was carried by fluorescence and circular dichroism (CD) measurements at 25 and 37° C. Also, cytotoxicity and apoptotic activity of the complex were studied against cancer model cell line of K562 using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Fluorescence data revealed that the intrinsic fluorescence of β-CN was quenched by the addition of Pt(II) complex through dynamic quenching mechanism. The CD spectra indicated that the binding of Pt(II) complex to β-CN causes a slight conformational change in the secondary structure of protein. Also, MTT assay represented growth inhibitory effect of the complex toward the cancer cell line. From above results, it can be concluded that β-CN can bind to the Pt complex and transfer this new anticancer drug. It may be suggested that the antitumor activity of this complex against K562 cell reveals typical morphology features of apoptotic death.
在本研究中，研究了β-酪蛋白（β-CN）与一种新合成的作为抗癌化合物的铂（II）配合物（联吡啶吗啉二硫代氨基甲酸盐铂（II）硝酸盐）之间的相互作用。该研究通过在25和37°C下进行荧光和圆二色性（CD）测量来进行。此外，使用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）法研究了该配合物对K562癌症模型细胞系的细胞毒性和凋亡活性。荧光数据表明，通过动态猝灭机制，添加铂（II）配合物可猝灭β-CN的固有荧光。CD光谱表明，铂（II）配合物与β-CN的结合会导致蛋白质二级结构发生轻微构象变化。此外，MTT法显示了该配合物对癌细胞系的生长抑制作用。从上述结果可以得出结论，β-CN可以与铂配合物结合并转运这种新的抗癌药物。可以推测，该配合物对K562细胞的抗肿瘤活性揭示了凋亡死亡的典型形态特征。
