Chen Xin, Mei Jie, Wu Junjie, Jing Jing, Ma Wenge, Zhang Jin, Dan Cheng, Wang Weimin, Gui Jian-Fang
College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Huazhong Agricultural University, Wuhan, 430070, China.
Mar Biotechnol (NY). 2015 Apr;17(2):190-8. doi: 10.1007/s10126-014-9607-7. Epub 2014 Nov 18.
Sex dimorphic growth pattern has significant theory and application implications in fish. Recently, a Y- and X-specific allele marker-assisted sex control technique has been developed for mass production of all-male population in yellow catfish (Pelteobagrus fulvidraco), but the genetic information for sex determination and sex control breeding has remained unclear. Here, we attempted to provide the first insight into a comprehensive transcriptome covering multiple tissues from XX females, XY males, and YY super-males of yellow catfish by using 454 GS-FLX platform, for a better assembly and gene coverage. A total of 1,202,933 high quality reads (about 540 Mbp) were obtained and assembled into 28,297 contigs and 141,951 singletons. BLASTX searches against the NCBI non-redundant protein database (nr) led a total of 52,564 unique sequences including 18,748 contigs and 33,816 singletons to match 25,669 known or predicted unique proteins. All of them with annotated function were categorized by gene ontology (GO) analysis, and 712 were assigned to reproduction and reproductive process. Some potential genes relevant to reproductive system including steroid hormone biosynthesis and GnRH (gonadotropin-releasing hormone) signaling pathway were further identified by Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis; and at least 21 sex determination and differentiation-related genes, such as Dmrt1, Sox9a/b, Cyp19b, WT1, and AMH were identified and characterized. Additionally, a total of 82,794 simple sequence repeats (SSRs), 26,450 single nucleotide polymorphisms (SNPs), and 4,145 insertions and deletions (INDELs) were revealed from the transcriptome data. Therefore, the current transcriptome resources highlight further studies on sex-control breeding in yellow catfish and will benefit future studies on reproduction and sex determination in teleost fish.
性别二态生长模式在鱼类中具有重要的理论和应用意义。最近，一种Y和X特异性等位基因标记辅助性别控制技术已被开发用于黄颡鱼（Pelteobagrus fulvidraco）全雄群体的大规模生产，但性别决定和性别控制育种的遗传信息仍不清楚。在此，我们试图通过使用454 GS-FLX平台，首次深入了解黄颡鱼XX雌性、XY雄性和YY超雄鱼多个组织的综合转录组，以实现更好的组装和基因覆盖。共获得1,202,933条高质量 reads（约540 Mbp），并组装成28,297个重叠群和141,951个单拷贝序列。对NCBI非冗余蛋白质数据库（nr）进行BLASTX搜索，共有52,564个独特序列（包括18,748个重叠群和33,816个单拷贝序列）匹配到25,669个已知或预测的独特蛋白质。所有具有注释功能的序列通过基因本体（GO）分析进行分类，其中712个被归类到生殖和生殖过程。通过京都基因与基因组百科全书（KEGG）分析进一步鉴定了一些与生殖系统相关的潜在基因，包括类固醇激素生物合成和GnRH（促性腺激素释放激素）信号通路；并鉴定和表征了至少21个与性别决定和分化相关的基因，如Dmrt1、Sox9a/b、Cyp19b、WT1和AMH。此外，从转录组数据中揭示了总共82,794个简单序列重复（SSR）、26,450个单核苷酸多态性（SNP）和4,145个插入缺失（INDEL）。因此，当前的转录组资源突出了对黄颡鱼性别控制育种的进一步研究，并将有利于未来对硬骨鱼生殖和性别决定的研究。
