Jameie Seyed Behnamedin, Masoumipoor Masoumeh, Janzadeh Atousa, Nasirinezhad Farinaz, Kerdari Mahdieh, Soleimani Maryam
1. Associate Professor of Anatomy and Neuroscience, Department of Medical Basic Sciences, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran.
2. MSc, Department of Nuclear Engineering, Sciences and Researches branch, Islamic Azad University, Tehran, Iran.
Med J Islam Repub Iran. 2014 Jul 13;28:58. eCollection 2014.
Neuropathic pain (NP) is one of the most suffering medical conditions that often fail to respond to certain pain therapy. Although its exact etiology is still unknown the role of reactive oxygen species (ROS) and oxidative stress were explored by many researchers. Neuropathies either central or peripheral lead to painful condition as well as social and economic isolation, thus various therapies were used to treat or reduce the pain. Laser therapy and antioxidant drugs have separately considered as treatment for NP, but the combination of them have not been used yet. In order to study the combination effects of Low Level Laser Therapy (LLLT) and Coenzyme Q10 (CoQ10) the present study was designed.
Sixty adult male rats (230-320g) were used in this experimental study that divided into six groups (n=10). Chronic constriction injury (CCI) was used to induce neuropathic pain. The CoQ10 or vehicle, a low level laser of 980nm was used for two consecutive weeks. Thermal and mechanical paw withdrawal thresholds were assessed before and after surgery on 7(th) and 14(th) days.
As we expected CCI decreased the pain threshold, whereas CoQ10 administration for two weeks increased mechanical and thermal threshold. The same results obtained for laser therapy using the CCI animals. Combination of laser 980nm with CoQ10 also showed significant differences in CCI animals.
Based on our findings the combination of CoQ10 with LLLT showed better effects than each one alone. In this regard we believe that there might be cellular and molecular synergism in simultaneous use of CoQ10 and LLLT on pain relief.
神经性疼痛（NP）是最令人痛苦的病症之一，通常对某些疼痛治疗无反应。尽管其确切病因仍不清楚，但许多研究人员探讨了活性氧（ROS）和氧化应激的作用。中枢或周围神经病变都会导致疼痛状况以及社会和经济隔离，因此人们使用了各种疗法来治疗或减轻疼痛。激光疗法和抗氧化药物已分别被视为NP的治疗方法，但尚未使用它们的联合疗法。为了研究低强度激光疗法（LLLT）和辅酶Q10（CoQ10）的联合作用，设计了本研究。
本实验研究使用60只成年雄性大鼠（230 - 320克），分为六组（n = 10）。采用慢性压迫损伤（CCI）诱导神经性疼痛。连续两周使用CoQ10或赋形剂、980nm的低强度激光。在第7天和第14天手术前后评估热和机械性缩爪阈值。
正如我们所预期的，CCI降低了疼痛阈值，而给予CoQ10两周则提高了机械和热阈值。对使用CCI动物进行激光治疗也得到了相同的结果。980nm激光与CoQ10联合使用在CCI动物中也显示出显著差异。
基于我们的研究结果，CoQ10与LLLT联合使用的效果优于单独使用任何一种。在这方面，我们认为CoQ10和LLLT同时使用在缓解疼痛方面可能存在细胞和分子协同作用。
