Alidadiyani Neda, Salehi Roya, Ghaderi Shahrooz, Samadi Nasser, Davaran Soodabeh
a Drug Applied Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.
b Department of Biochemistry and Clinical Laboratories , Faculty of Medical Sciences, Tabriz University of Medical Sciences , Tabriz , Iran.
Artif Cells Nanomed Biotechnol. 2016;44(2):603-9. doi: 10.3109/21691401.2014.975235. Epub 2014 Nov 18.
In this study, the ability of methotrexate (MTX)-loaded stimuli-responsive novel silica nanocomposites (MSNs) (with mean diameter of ± 60 nm) in the induction of apoptosis, and change in the Bax/Bcl-2 mRNA levels, were investigated. MTT assay and RT -PCR analysis were performed on MDA-MB-231 breast cancer cells, to evaluate their anti-proliferative, apoptotic and anti-apoptotic effects. MTX-loaded MSNs caused marked decrease in the percentage of viable cells, with a significant down-regulation in the level of expression of the anti-apoptotic gene (Bcl-2), and up-regulation in the apoptotic gene (Bax). MTX-loaded MSNs increased the efficacy of the chemotherapeutic agents in the inhibition of cell proliferation and induction of apoptosis.
在本研究中，研究了负载甲氨蝶呤（MTX）的刺激响应性新型二氧化硅纳米复合材料（MSNs）（平均直径±60nm）诱导细胞凋亡的能力以及Bax/Bcl-2 mRNA水平的变化。对MDA-MB-231乳腺癌细胞进行MTT法检测和RT-PCR分析，以评估其抗增殖、促凋亡和抗凋亡作用。负载MTX的MSNs导致活细胞百分比显著降低，抗凋亡基因（Bcl-2）表达水平显著下调，凋亡基因（Bax）上调。负载MTX的MSNs提高了化疗药物抑制细胞增殖和诱导细胞凋亡的功效。
