Tonogai Ichiro, Takahashi Mitsuhiko, Yukata Kiminori, Sato Ryosuke, Nikawa Takeshi, Yasui Natsuo, Sairyo Koichi
aDepartment of Orthopedics bDepartment of Nutritional Physiology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan.
J Pediatr Orthop B. 2015 Mar;24(2):162-9. doi: 10.1097/BPB.0000000000000117.
The aim of this study was to determine whether osteoactivin attenuated skeletal muscle fibrosis caused by distraction osteogenesis. Tibial osteotomies were performed on wild-type and osteoactivin-transgenic (OA-Tg) mice, and tibiae were distracted for 2 weeks. Ankle plantar flexion torque and the gastrocnemius muscles were analyzed. The amount and area of collagenous tissue and the passive torque were reduced in the OA-Tg group at 8 weeks after osteotomy. Transcript levels of matrix metalloprotease (mmp)-3 and MMP-9 were upregulated, and MMP-3 and MMP-9 proteins were increased in the OA-Tg group. Osteoactivin-mediated increase in MMPs may attenuate skeletal muscle fibrosis.
本研究的目的是确定骨激活素是否能减轻牵张成骨引起的骨骼肌纤维化。对野生型和骨激活素转基因（OA-Tg）小鼠进行胫骨截骨术，并对胫骨进行2周的牵张。分析踝关节跖屈扭矩和腓肠肌。截骨术后8周，OA-Tg组的胶原组织数量和面积以及被动扭矩均降低。基质金属蛋白酶（mmp）-3和MMP-9的转录水平上调，且OA-Tg组中MMP-3和MMP-9蛋白增加。骨激活素介导的基质金属蛋白酶增加可能减轻骨骼肌纤维化。
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