Tonogai Ichiro, Hayashi Fumio, Iwame Toshiyuki, Takasago Tomoya, Matsuura Tetsuya, Sairyo Koichi
Department of Orthopedics, Institute of Health Biosciences, Tokushima University Graduate School, 3-18-15 Kuramoto, Tokushima, 770-8503, Japan.
J Exp Orthop. 2018 Jul 13;5(1):26. doi: 10.1186/s40634-018-0143-7.
Skeletal muscle fibrosis caused by an increase in collagen deposition often occurs after distraction osteogenesis. Although studies are available reporting the effects of platelet-rich plasma (PRP) on tissue healing following injury, current findings remain controversial. This study focused on determining whether PRP reduces skeletal muscle fibrosis caused by distraction osteogenesis.
Tibial osteotomies were performed on 8-week-old wild type mice, and tibiae were distracted at a rate of 0.42 mm/day for 2 weeks, starting 1 week after osteotomy. Immediately after distraction was completed (3 weeks after osteotomy), PRP or phosphate buffered saline (as a sham) was injected into the gastrocnemius (GC) muscle. The GC muscles were harvested and analyzed.
The amount and area of collagenous tissue increased in both the PRP and control groups following distraction osteogenesis, but the changes were not significantly different between both groups at all time points (p = 0.89, 0.45, 0.33 and 0.52 at 4, 6, 8 and 10 weeks).
From this study, our results suggest that PRP did not significantly reduce skeletal muscle fibrosis due to distraction osteogenesis.
牵张成骨后常因胶原蛋白沉积增加而导致骨骼肌纤维化。尽管已有研究报道富血小板血浆（PRP）对损伤后组织愈合的影响，但目前的研究结果仍存在争议。本研究聚焦于确定PRP是否能减轻牵张成骨引起的骨骼肌纤维化。
对8周龄野生型小鼠进行胫骨截骨术，截骨术后1周开始，以每天0.42毫米的速度对胫骨进行牵张，持续2周。牵张完成后（截骨术后3周），立即将PRP或磷酸盐缓冲盐水（作为对照）注射到腓肠肌（GC）中。然后采集并分析GC肌肉。
牵张成骨后，PRP组和对照组的胶原组织数量和面积均增加，但在所有时间点两组之间的变化均无显著差异（4、6、8和10周时p分别为0.89、0.45、0.33和0.52）。
从本研究来看，我们的结果表明PRP并不能显著减轻牵张成骨所致的骨骼肌纤维化。
