Werner J A, Schünke M
Department of Ear, Nose, and Throat Surgery, University of Kiel, FRG.
Acta Histochem. 1989;85(1):15-21. doi: 10.1016/S0065-1281(89)80090-X.
Lymphatic capillaries with narrow lumina cannot be identified with certainty by means of conventional light microscopy. This, however, can be achieved with great precision by means of labelling the 5'-nucleotidase activity which is high in lymphatic capillaries. In blood capillaries, on the other hand, it is either lacking or much less pronounced depending on the organ. The reactions performed thus far occurred primarily in the presence of lead ions which had a toxic effect on the enzymes. In cerium, however, this inhibiting influence can be neglected. 2 light-microscopic procedures of labelling the 5'-nucleotidase activity, which make use of cerium, are presented and compared here for the first time. The cerium perhydroxide technique is especially suited for demonstrating the lymphatic capillaries. In comparison to the previous enzyme histochemical methods, a definitely more precise demonstration of the endothelia of small lymphatics now is possible. The reason for this is that this method only reacts to regions of high enzyme activity. As opposed to previous tests on cryostat sections, the lumen of the lymphatic capillary is nearly always identifiable as such if employing this type of reaction. These properties of the cerium-perhydroxide reaction allow definite statements regarding the distribution of the lymphatic capillaries in the tissue. Its simple and rapid course of reaction favour the use of this method in clinical problems. In the other technique presented, the manganese dioxide technique, the demonstration of lymphatic capillaries is by far less exact due to its greater sensitivity even to low enzyme activities.
管腔狭窄的毛细淋巴管无法通过传统光学显微镜确切识别。然而，通过标记毛细淋巴管中活性较高的5'-核苷酸酶，可以非常精确地实现这一点。另一方面，在毛细血管中，5'-核苷酸酶要么缺乏，要么根据器官不同而活性低得多。迄今为止进行的反应主要是在铅离子存在的情况下进行的，而铅离子对酶有毒性作用。然而，在铈存在的情况下，这种抑制作用可以忽略不计。本文首次介绍并比较了两种利用铈标记5'-核苷酸酶活性的光学显微镜方法。氢氧化铈技术特别适合于显示毛细淋巴管。与以前的酶组织化学方法相比，现在可以更精确地显示小淋巴管的内皮。原因是这种方法只对高酶活性区域起反应。与以前在低温切片上的测试不同，如果采用这种反应类型，几乎总能识别出毛细淋巴管的管腔。氢氧化铈反应的这些特性使得能够明确说明组织中毛细淋巴管的分布情况。其简单快速的反应过程有利于该方法在临床问题中的应用。在介绍的另一种技术——二氧化锰技术中，由于其对低酶活性甚至更高的敏感性，显示毛细淋巴管的效果远不如前者精确。
