de Alfonso Ignacio, Vacas Sandra, Primo Jaime
Ecologı́a y Protección Agrı́cola, S.L., Apartado de Correos no. 50, 46240 Carlet, Spain.
J Agric Food Chem. 2014 Dec 10;62(49):11976-9. doi: 10.1021/jf504821a. Epub 2014 Dec 1.
The influence of α-copaene as a fruit volatile in the susceptibility of Olea europaea L. to the olive fruit fly Bactrocera oleae (Rossi) has been investigated. By studies on the relative area of volatile components from different cultivars, a positive correlation was found between the abundance of α-copaene in the samples and the corresponding degree of fruit infestation. SPME-GC-MS analysis of volatiles from uninfested fruits of O. europaea L. cv. Serrana were performed over two years to determine the variation of α-copaene throughout the different phenological stages. The results suggested that this sesquiterpene has a significant effect on cultivar susceptibility and may act as an oviposition promoter. Further analysis by chiral GC showed that olive fruits release both α-copaene enantiomers. Bioassays on each enantiomer revealed that fruits with increased amounts of (+)-α-copaene favor oviposition of B. oleae females, whereas the increase of (-)-α-copaene affords no statistically significant differences in host preference.
研究了α-可巴烯作为一种果实挥发物对油橄榄（Olea europaea L.）对橄榄实蝇（Bactrocera oleae (Rossi)）易感性的影响。通过对不同品种挥发成分相对面积的研究，发现样品中α-可巴烯的含量与相应的果实侵染程度呈正相关。对油橄榄品种塞拉纳（O. europaea L. cv. Serrana）未受侵染果实的挥发物进行了两年的固相微萃取-气相色谱-质谱（SPME-GC-MS）分析，以确定α-可巴烯在不同物候期的变化。结果表明，这种倍半萜对品种易感性有显著影响，可能作为产卵促进剂。手性气相色谱进一步分析表明，橄榄果实释放两种α-可巴烯对映体。对每种对映体的生物测定表明，(+)-α-可巴烯含量增加的果实有利于橄榄实蝇雌虫产卵，而(-)-α-可巴烯含量增加在寄主偏好方面没有统计学上的显著差异。
