Borassi Cecilia, Di Giorgio Juliana Pérez, Scarpin María R, Muschietti Jorge, Estevez José M
Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-CONICET), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
Methods Mol Biol. 2015;1242:41-7. doi: 10.1007/978-1-4939-1902-4_3.
Pollen tubes elongate by tip growth toward the ovule to deliver the sperm cells during fertilization. Since pollen tubes from several species can be grown in vitro maintaining their polarity, pollen tube growth is a suitable model system to study cell polarity and tip growth. A. thaliana pollen tubes germinated in vitro for 6 h can reach up to 800 μm. By studying the phenotype of mutants of T-DNA insertion lines, genes involved in pollen tube growth can be identified. Moreover, components involved in the regulation of pollen tube growth such as calcium ions and reactive oxygen species (ROS) can be analyzed.
花粉管通过顶端生长向胚珠延伸，以便在受精过程中输送精子细胞。由于来自多个物种的花粉管可以在体外生长并保持其极性，花粉管生长是研究细胞极性和顶端生长的合适模型系统。在体外萌发6小时的拟南芥花粉管可长达800微米。通过研究T-DNA插入系突变体的表型，可以鉴定出参与花粉管生长的基因。此外，还可以分析参与花粉管生长调控的成分，如钙离子和活性氧（ROS）。
