Zambrowicz Aleksandra, Pokora Marta, Setner Bartosz, Dąbrowska Anna, Szołtysik Marek, Babij Konrad, Szewczuk Zbigniew, Trziszka Tadeusz, Lubec Gert, Chrzanowska Józefa
Department of Animal Products Technology and Quality Management, Faculty of Food Science, Wrocław University of Environmental and Life Sciences, Chełmońskiego 37/41, 51-630, Wrocław, Poland,
Amino Acids. 2015 Feb;47(2):369-80. doi: 10.1007/s00726-014-1869-x. Epub 2014 Nov 20.
An egg yolk protein by-product following ethanol extraction of phospholipids (YP) was hydrolyzed with pepsin to produce and identify novel peptides that revealed antioxidant, ACE inhibitory and antidiabetic (α-glucosidase and DPP-IV inhibitory) activities. The peptic hydrolysate of YP was fractionated by ion-exchange chromatography and reversed-phase high-pressure liquid chromatography. Isolated peptides were identified using mass spectrometry (MALDI-ToF) and the Mascot Search Results database. Four peptides of MW ranging from 1,210.62 to 1,677.88 Da corresponded to the fragments of Apolipoprotein B (YINQMPQKSRE; YINQMPQKSREA), Vitellogenin-2 (VTGRFAGHPAAQ) and Apovitellenin-1 (YIEAVNKVSPRAGQF). These peptides were chemically synthesized and showed antioxidant, ACE inhibitory or/and antidiabetic activities. Peptide YIEAVNKVSPRAGQF exerted the strongest ACE inhibitory activity, with IC50 = 9.4 µg/mL. The peptide YINQMPQKSRE showed the strongest DPPH free radical scavenging and DPP-IV inhibitory activities and its ACE inhibitory activity (IC50) reached 10.1 µg/mL. The peptide VTGRFAGHPAAQ revealed the highest α-glucosidase inhibitory activity (IC50 = 365.4 µg/mL). A novel nutraceutical effect for peptides from an egg yolk hydrolysate was shown.
在对磷脂进行乙醇提取后得到的蛋黄蛋白副产物（YP）用胃蛋白酶水解，以产生并鉴定具有抗氧化、ACE抑制和抗糖尿病（α-葡萄糖苷酶和DPP-IV抑制）活性的新型肽。YP的胃蛋白酶水解产物通过离子交换色谱和反相高压液相色谱进行分离。使用质谱（MALDI-ToF）和Mascot搜索结果数据库鉴定分离出的肽。四种分子量在1210.62至1677.88 Da之间的肽对应于载脂蛋白B（YINQMPQKSRE；YINQMPQKSREA）、卵黄生成素-2（VTGRFAGHPAAQ）和卵黄磷蛋白-1（YIEAVNKVSPRAGQF）的片段。这些肽经过化学合成，并表现出抗氧化、ACE抑制或/和抗糖尿病活性。肽YIEAVNKVSPRAGQF表现出最强的ACE抑制活性，IC50 = 9.4 µg/mL。肽YINQMPQKSRE表现出最强的DPPH自由基清除和DPP-IV抑制活性，其ACE抑制活性（IC50）达到10.1 µg/mL。肽VTGRFAGHPAAQ显示出最高的α-葡萄糖苷酶抑制活性（IC50 = 365.4 µg/mL）。展示了蛋黄水解产物中肽的一种新型营养保健作用。
