抗辅酶吡咯喹啉醌抗体的产生。

Production of antibodies against the coenzyme pyrrolequinoline quinone.

作者信息

Citro G, Verdina A, Galati R, Floris G, Sabatini S, Finazzi-Agrò A

机构信息

Istituto Regina Elena per la Ricerca sul Cancro, Roma, Italy.

出版信息

FEBS Lett. 1989 Apr 24;247(2):201-4. doi: 10.1016/0014-5793(89)81334-1.

DOI:10.1016/0014-5793(89)81334-1

PMID:2541012

Abstract

Polyclonal antibodies against pyrrolequinoline quinone have been elicited in rabbits. These antibodies react with free and protein-bound pyrrolequinoline quinone. In particular they react with native and denatured lentil seedling amine oxidase as detected by dot-blot and ELISA assays. The presence of 1 mol pyrrolequinoline quinone per mol of enzyme was determined by the last method.

摘要

已在兔子体内诱发了针对吡咯喹啉醌的多克隆抗体。这些抗体可与游离的和与蛋白质结合的吡咯喹啉醌发生反应。特别是，通过斑点印迹法和酶联免疫吸附测定法检测发现，它们可与天然的和变性的小扁豆幼苗胺氧化酶发生反应。通过后一种方法确定每摩尔酶中含有1摩尔吡咯喹啉醌。