Hoosein N M, Thim L, Jørgensen K H, Brattain M G
Bristol-Baylor Laboratory, Department of Pharmacology, Baylor College of Medicine, Houston, TX 77030.
FEBS Lett. 1989 Apr 24;247(2):303-6. doi: 10.1016/0014-5793(89)81357-2.
The effects of a novel polypeptide, pancreatic spasmolytic polypeptide (PSP) on a colon carcinoma cell line (HCT 116) were examined. PSP stimulated the incorporation of [3H]thymidine into HCT 116 cells as well as cell proliferation in a dose-dependent manner. Maximal increase in [3H]thymidine incorporation of 50-60% occurred at 3-300 microM PSP. The VIP-mediated-increase in cAMP levels was reduced by PSP at greater than 1 microM concentrations. PSP is highly homologous to the estrogen-induced pS2 protein in MCF-7 breast cancer cells. We find that PSP also enhanced [3H]thymidine incorporation in MCF-7 cells. These findings indicate for the first time that PSP has growth stimulatory properties.
研究了一种新型多肽——胰腺解痉多肽（PSP）对结肠癌细胞系（HCT 116）的作用。PSP以剂量依赖的方式刺激[3H]胸苷掺入HCT 116细胞以及细胞增殖。在3 - 300微摩尔/升的PSP浓度下，[3H]胸苷掺入量最大增加50 - 60%。当浓度大于1微摩尔/升时，PSP可降低VIP介导的cAMP水平升高。PSP与MCF - 7乳腺癌细胞中雌激素诱导的pS2蛋白高度同源。我们发现PSP也能增强MCF - 7细胞中[3H]胸苷的掺入。这些发现首次表明PSP具有生长刺激特性。
