Itoh H, Tomita M, Uchino H, Kobayashi T, Kataoka H, Sekiya R, Nawa Y
Department of Parasitology, Miyazaki Medical College, Japan.
Biochem J. 1996 Sep 15;318 ( Pt 3)(Pt 3):939-44. doi: 10.1042/bj3180939.
By using a combination of the methods of reverse transcription-PCR and rapid amplification of cDNA ends, a cDNA for rat pS2 peptide (rpS2) was successfully cloned and sequenced from rat stomach. By RNA blot analysis, the gene was shown to be expressed abundantly in the stomach and only faintly in the duodenum, but not in other tissues including the distal small and large intestines. rpS2 expression was also examined in the rectum during the course of acetic acid-induced colitis; rpS2 mRNA was detected during the acute phase of colitis but not in normal controls or during the recovery phase. On the other hand, expression of rat intestinal trefoil factor (rITF) was down-regulated during the acute phase of colitis and then up-regulated during the recovery phase, whereas rat spasmolytic peptide was not detectable throughout the course of the induced colitis. These results indicate that the patterns and timing of the expression of these trefoil peptides are different from each other. rpS2 may play an important role in the proliferation of intestinal epithelial cells during the acute phase of mucosal ulceration, whereas rITF may be involved in differentiation of the cells, particularly to form goblet cells, during the recovery phase.
通过结合逆转录 - PCR和cDNA末端快速扩增方法，成功从大鼠胃中克隆并测序了大鼠pS2肽（rpS2）的cDNA。通过RNA印迹分析表明，该基因在胃中大量表达，在十二指肠中表达微弱，但在包括远端小肠和大肠在内的其他组织中不表达。还检测了乙酸诱导的结肠炎过程中直肠中的rpS2表达；在结肠炎急性期检测到rpS2 mRNA，但在正常对照或恢复期未检测到。另一方面，大鼠肠三叶因子（rITF）的表达在结肠炎急性期下调，然后在恢复期上调，而在整个诱导性结肠炎过程中均未检测到大鼠解痉肽。这些结果表明，这些三叶肽的表达模式和时间彼此不同。rpS2可能在黏膜溃疡急性期肠上皮细胞增殖中起重要作用，而rITF可能在恢复期参与细胞分化，特别是形成杯状细胞。
